In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies
| Autor: | Mónica Carril, Mona Atabakhshi-Kashi, Carolina Carrillo-Carrión, Wolfgang J. Parak, Hossein Mahdavi, Khosro Khajeh |
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| Přispěvatelé: | Teerthanker Mahaveer University, Iranian Government, German Research Foundation, Universidad de Sevilla. Departamento de Química Inorgánica, Taipei Medical University (TMU), Biotechnology Development Council. Islamic Republic of Iran, Advanced Imaging of Matter (CUI). Alemania |
| Rok vydání: | 2021 |
| Předmět: |
General Chemical Engineering
media_common.quotation_subject education Nanoparticle quantum dots antibody conjugation 02 engineering and technology 010402 general chemistry Endocytosis 01 natural sciences Article Flow cytometry law.invention Hydrophobic effect Confocal microscopy law medicine General Materials Science Internalization QD1-999 media_common medicine.diagnostic_test Chemistry self-assembly 021001 nanoscience & nanotechnology Fluorescence In vitro fluorination 0104 chemical sciences Biophysics nanoparticles 0210 nano-technology |
| Zdroj: | Addi. Archivo Digital para la Docencia y la Investigación instname Nanomaterials Nanomaterials, Vol 11, Iss 1906, p 1906 (2021) Volume 11 Issue 8 |
| Popis: | Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine-fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly). This research was financially supported by the research core of TMU (Grant No: IG-39707) and the Biotechnology Development Council of the Islamic Republic of Iran (Grant No: 950709). Part of this work was funded by the Cluster of Excellence ‘Advanced Imaging of Matter’ of the Deutsche Forschungsgemeinschaft (DFG)-EXC 2056-project ID 390715994. |
| Databáze: | OpenAIRE |
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