Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

Autor:	Morten T. Venø, Jesper Just, Tingting Gu, Peter Sieljacks, Kim Ryun Drasbek, Frank Vincenzo de Paoli, Jens R. Nyengaard, Kristian Vissing, Jean Farup, Malene Jørgensen, Rikke Bæk, Jørgen Kjems, Mette Sloth, Yan Yan
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Male Physiology Blotting Western Muscle Proteins lcsh:Medicine Article Muscle hypertrophy Biological pathway Extracellular Vesicles Young Adult Microscopy Electron Transmission Precursor cell microRNA medicine Humans Muscle Skeletal lcsh:Science Multidisciplinary Molecular medicine Chemistry lcsh:R Protein turnover High-Throughput Nucleotide Sequencing Skeletal muscle Resistance Training Blood flow Flow Cytometry Cell biology MicroRNAs medicine.anatomical_structure Regional Blood Flow lcsh:Q Cell activation
Zdroj:	Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) Just, J, Yan, Y, Farup, J, Sieljacks, P, Sloth, M, Venø, M, Gu, T, de Paoli, F V, Nyengaard, J R, Bæk, R, Jørgensen, M M, Kjems, J, Vissing, K & Drasbek, K R 2020, ' Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles ', Scientific Reports, vol. 10, no. 1, 5835, pp. 1-13 . https://doi.org/10.1038/s41598-020-62456-3 Just, J, Yan, Y, Farup, J, Sieljacks, P, Sloth, M, Venø, M, Gu, T, de Paoli, F V, Nyengaard, J R, Bæk, R, Jørgensen, M M, Kjems, J, Vissing, K & Drasbek, K R 2020, ' Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles ', Scientific Reports, vol. 10, no. 1, 5835 . https://doi.org/10.1038/s41598-020-62456-3 Scientific Reports
ISSN:	2045-2322
DOI:	10.1038/s41598-020-62456-3
Popis:	Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a37e619f1e522468e7f9aced5c65e848 http://link.springer.com/article/10.1038/s41598-020-62456-3 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.