Reduced Graphene Oxides Modulate the Expression of Cell Receptors and Voltage-Dependent Ion Channel Genes of Glioblastoma Multiforme

Autor: Jarosław Szczepaniak, Marta Grodzik, Olga Witkowska-Piłaszewicz, Barbara Strojny-Cieslak, Karolina Daniluk, Anna Sobczyk-Guzenda, Sławomir Jaworski, Joanna Jagiełło, Maciej Szmidt, Mateusz Wierzbicki, Dorota Nowak, Malwina Sosnowska
Rok vydání: 2020
Předmět:
Cells
Receptors
Cell Surface
reduced graphene oxide
Catalysis
Ion Channels
Article
law.invention
Membrane Potentials
lcsh:Chemistry
Inorganic Chemistry
Cell membrane
glioblastoma multiforme
law
Chloride Channels
Cell Line
Tumor
Spectroscopy
Fourier Transform Infrared
Extracellular
medicine
Humans
RNA
Messenger
Physical and Theoretical Chemistry
Receptor
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Ion channel
Membrane potential
Voltage-gated ion channel
Chemistry
Graphene
Organic Chemistry
graphene
Membrane Proteins
General Medicine
Computer Science Applications
Gene Expression Regulation
Neoplastic
Membrane
medicine.anatomical_structure
lcsh:Biology (General)
lcsh:QD1-999
Potassium Channels
Voltage-Gated
cell membrane receptor
Biophysics
Microscopy
Electron
Scanning
Graphite
membrane potential
Glioblastoma
Oxidation-Reduction
voltage-gated ion channel
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 2
International Journal of Molecular Sciences, Vol 22, Iss 515, p 515 (2021)
ISSN: 1422-0067
Popis: The development of nanotechnology based on graphene and its derivatives has aroused great scientific interest because of their unusual properties. Graphene (GN) and its derivatives, such as reduced graphene oxide (rGO), exhibit antitumor effects on glioblastoma multiforme (GBM) cells in vitro. The antitumor activity of rGO with different contents of oxygen-containing functional groups and GN was compared. Using FTIR (fourier transform infrared) analysis, the content of individual functional groups (GN/exfoliation (ExF), rGO/thermal (Term), rGO/ammonium thiosulphate (ATS), and rGO/ thiourea dioxide (TUD)) was determined. Cell membrane damage, as well as changes in the cell membrane potential, was analyzed. Additionally, the gene expression of voltage-dependent ion channels (clcn3, clcn6, cacna1b, cacna1d, nalcn, kcne4, kcnj10, and kcnb1) and extracellular receptors was determined. A reduction in the potential of the U87 glioma cell membrane was observed after treatment with rGO/ATS and rGO/TUD flakes. Moreover, it was also demonstrated that major changes in the expression of voltage-dependent ion channel genes were observed in clcn3, nalcn, and kcne4 after treatment with rGO/ATS and rGO/TUD flakes. Furthermore, the GN/ExF, rGO/ATS, and rGO/TUD flakes significantly reduced the expression of extracellular receptors (uPar, CD105) in U87 glioblastoma cells. In conclusion, the cytotoxic mechanism of rGO flakes may depend on the presence and types of oxygen-containing functional groups, which are more abundant in rGO compared to GN.
Databáze: OpenAIRE
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