Deep hypothermia impact on acid–base parameters and liver antioxidant status in an in vivo rat model
| Autor: | Norma Alva, Teresa Carbonell, J. Palomeque |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Physiology Hemodynamics Blood Pressure medicine.disease_cause Antioxidants Body Temperature Rats Sprague-Dawley Superoxide dismutase chemistry.chemical_compound Hypothermia Induced Physiology (medical) Internal medicine Heart rate medicine Animals Lactic Acid Rewarming Acid-Base Equilibrium Pharmacology chemistry.chemical_classification biology Glutathione peroxidase Alanine Transaminase General Medicine Glutathione Carbon Dioxide Hypothermia Hypoxia (medical) Cell Hypoxia Rats Oxygen Endocrinology Liver Biochemistry chemistry Blood Circulation biology.protein Blood Gas Analysis medicine.symptom Reactive Oxygen Species Oxidation-Reduction Oxidative stress |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 87:471-478 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/y09-033 |
| Popis: | Although clinical hypothermia is used for reducing postischemic damage, injurious effects have also been reported. To determine whether hypoxia and oxidative stress are induced by systemic deep hypothermia, we used an in vivo rat model keeping the arterial Pco2constant. Animals were divided into 4 groups: sham, 2 h deep hypothermia (21 °C), 1 h posthypothermia (rewarmed to 37 °C after 2 h deep hypothermia), and 3 h normothermia. Blood gases, portal vein blood flow, arterial pressure, and heart rate were monitored throughout the experiment. Liver enzyme antioxidant activity was also examined. The hemodynamic parameters decreased drastically during hypothermia, but were fully restored after rewarming. No changes in hepatic antioxidant activity (catalase, glutathione peroxidase, and superoxide dismutase) were observed. The redox level in liver (GSH/GSSG ratio) was preserved in hypothermia but decreased when animals were rewarmed. ALT did not increase and no evidence of tissue hypoxia was detected in liver regarding the restricted flow during hypothermia. With the described protocol, deep hypothermia is regarded as an experimental safe model. |
| Databáze: | OpenAIRE |
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