Davallia bilabiata Inhibits TNF-α-Induced Adhesion Molecules and Chemokines by Suppressing IKK/NF-kappa B Pathway in Vascular Endothelial Cells
| Autor: | Hsiao-Ting Wu, Cheng-Chieh Chang, Jer-Ming Sheen, Rong-Chi Yang, Sheng-Teng Huang, Jong-Hwei S. Pang |
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| Rok vydání: | 2014 |
| Předmět: |
Umbilical Veins
Chemokine Anti-Inflammatory Agents IκB kinase Biology Monocytes Arthritis Rheumatoid Osteoarthritis Cell Adhesion Humans Cytotoxic T cell CX3CL1 Cells Cultured Cell Nucleus Dose-Response Relationship Drug Plant Extracts Tumor Necrosis Factor-alpha Cell adhesion molecule NF-kappa B Endothelial Cells General Medicine NFKB1 I-kappa B Kinase Cell biology IκBα Complementary and alternative medicine Ferns biology.protein Female Signal transduction Cell Adhesion Molecules Phytotherapy Signal Transduction |
| Zdroj: | The American Journal of Chinese Medicine. 42:1411-1429 |
| ISSN: | 1793-6853 0192-415X |
| DOI: | 10.1142/s0192415x1450089x |
| Popis: | Davallia bilabiata (D. bilabiata) is also called GuSuiBu in Taiwan and is used as a substitute for Drynaria fortunei J. Sm. It is often used for trauma and bone repair. The inhibitory effect of D. bilabiata on inflammatory activity has not been reported. In the present study, we aimed to study the mechanism of anti-inflammation of D. bilabiata on the adhesion of leukocytes to vascular endothelial cells. The results showed that D. bilabiata, at concentrations without cytotoxic effect, inhibited the adhesion of monocytes (THP-1) to the TNF-α-stimulated human umbilical vascular endothelial cells (HUVECs). D. bilabiata suppressed the expression of the adhesion molecules ICAM, VCAM, and E-selectin at both the mRNA and protein level. In addition, both of the TNF-α-induced mRNA and protein expression of chemokines including fractalkine/CX3CL1, MCP-1 and RANTES as well as the level of secreted soluble fractalkine were decreased by D. bilabiata. We also verified that D. bilabiata inhibited the TNF-α-induced nuclear translocation of NF-κB through the inhibitory process on the TNF-α-activated phosphorylation of IKKα, IKKβ, IκB and NF-κB. All together, we concluded that the D. bilabiata affected the canonical pathway of TNF-α-induced NF-κB activation and down-regulated cell adhesion molecules and chemokine expression through inhibition of the NF-κB/IκBα/IKK signaling pathway. These findings strongly indicated that D. bilabiata might be a promising alternative/adjunct treatment for inflammatory diseases, such as rheumatoid arthritis and osteoarthritis. |
| Databáze: | OpenAIRE |
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