Apigenin Modulates Dendritic Cell Activities and Curbs Inflammation Via RelB Inhibition in the Context of Neuroinflammatory Diseases
Autor: | Zafar K. Khan, Mariana Bernui, Frank Bearoff, Patrick Moore, Raina Bhavsar, Mitzi Nagarkatti, Rashida Ginwala, Narendra P. Singh, Pooja Jain |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Encephalomyelitis Autoimmune Experimental T cell T-Lymphocytes Immunology Antigen presentation Neuroscience (miscellaneous) Lymphocyte Activation Article 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Immune system medicine Immunology and Allergy Animals Humans Apigenin Neuroinflammation Pharmacology Inflammation RELB Transcription Factor RelB FOXP3 Dendritic cell Dendritic Cells Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure chemistry Cancer research 030217 neurology & neurosurgery |
Zdroj: | J Neuroimmune Pharmacol |
ISSN: | 1557-1904 |
Popis: | Neuroinflammation leads to tissue injury causing many of the clinical symptoms of Multiple Sclerosis, an autoimmune disorder of the central nervous system (CNS). While T cells, specifically Th1 and Th17 cells, are the ultimate effectors of this disease, dendritic cells (DCs) mediate T cell polarization, activation, etc. In our previous study, Apigenin, a natural flavonoid, has been shown to reduce EAE disease severity through amelioration of demyelination in the CNS as well as the sequestering of DCs and other myeloid cells in the periphery. Here, we show that Apigenin exerts its effects possibly through shifting DC modulated T cell responses from Th1 and Th17 type towards Treg directed responses evident through the decrease in T-bet, IFN-γ (Th1), IL-17 (Th17) and increase in IL-10, TGF-β and FoxP3 (Treg) expression in cells from both normal human donors and EAE mice. RelB, an NF-κβ pathway protein is central to DC maturation, its antigen presentation capabilities and DC-mediated T cell activation. Apigenin reduced mRNA and protein levels of RelB and also reduced its nuclear translocation. Additionally, siRNA-mediated silencing of RelB further potentiated the RelB-mediated effects of Apigenin thus confirming its role in Apigenin directed regulation of DC biology. These results provide key information about the molecular events controlled by Apigenin in its regulation of DC activity marking its potential as a therapy for neuroinflammatory disease. Graphical Abstract. |
Databáze: | OpenAIRE |
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