Antheraea pernyi silk fibroin for targeted gene delivery of VEGF165-Ang-1 with PEI
| Autor: | Yanni Yu, Linlin Lv, Jicheng Yang, Yu Liu, Caili Ma, Renchuan You, Mingzhong Li |
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| Rok vydání: | 2014 |
| Předmět: |
Vascular Endothelial Growth Factor A
Materials science Silk Biomedical Engineering Fibroin Bioengineering macromolecular substances Antheraea pernyi Moths Gene delivery Transfection Cell Line Biomaterials Mice Nanocapsules Tissue engineering Absorbable Implants Materials Testing Angiopoietin-1 Animals Cytotoxicity Expression vector biology technology industry and agriculture Fibroblasts biology.organism_classification Molecular biology In vitro Gene Targeting Fibroins Plasmids |
| Zdroj: | Biomedical Materials. 9:035015 |
| ISSN: | 1748-605X 1748-6041 |
| DOI: | 10.1088/1748-6041/9/3/035015 |
| Popis: | Vascularization is a crucial challenge in tissue engineering. One solution for this problem is to implant scaffolds that contain functional genes that promote vascularization by providing angiogenic growth factors via a gene delivery carrier. Poly(ethylenimine) (PEI) is a gene delivery carrier with high transfection efficiency but with cytotoxicity. To solve this problem, we utilized Antheraea pernyi silk fibroin (ASF), which has favorable cytocompatibility and biodegradability, RGD sequences and a negative charge, in conjunction with PEI, as the delivery vector for vascular endothelial growth factor (VEGF) 165-angiopoietin-1 (Ang-1) dual gene simultaneous expression plasmid, creating an ASF/PEI/pDNA complex. The results suggested that the zeta potential of the ASF/PEI/pDNA complex was significantly lower than that of the PEI/pDNA complex. Decreased nitrogen and increased oxygen on the surface of the complex demonstrated that the ASF had successfully combined with the surface of the PEI/pDNA. Furthermore, the complexes resisted digestion by nucleic acid enzymes and degradation by serum. L929 cells were cultured and transfected in vitro and improved cytotoxicity was found when the cells were transfected with ASF/PEI/pDNA compared with PEI/pDNA. In addition, the transfection efficiency and VEGF secretion increased. In general, this study provides a novel method for decreasing the cytotoxicity of PEI gene delivery vectors and increasing transfection efficiency of angiogenesis-related genes. |
| Databáze: | OpenAIRE |
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