Contrast improvement in indocyanine green fluorescence sensing in thick tissue using a time-gating method
| Autor: | Goro Nishimura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science media_common.quotation_subject 01 natural sciences Signal Imaging phantom Article 010309 optics 03 medical and health sciences chemistry.chemical_compound Optics 0103 physical sciences Contrast (vision) 030304 developmental biology media_common 0303 health sciences business.industry Detector Fluorescence Atomic and Molecular Physics and Optics Photon counting chemistry business Indocyanine green Biotechnology |
| Popis: | Indocyanine green-based fluorescence imaging techniques are very powerful in clinical applications, but the imaging is restricted to the signal from the near-surface region of tissue. Here, we focus on the method to discriminate the fluorescence signal from the background using a time-domain gating technique. The contrast of the fluorescence image from a fluorescence object at more than 1 cm depth in a meat phantom could be enhanced about 4-5 times relative to the continuous wave method if the time-gate range was properly selected. Further, a Monte Carlo simulation with a simple background model indicates that a shorter source and detector distance is more effective to improve the contrast. The simple time-gating method will enable a highly sensitive fluorescence detection in thick tissue. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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