Sulfatide and Na+-K+-ATPase: a salinity-sensitive relationship in the gill basolateral membrane of rainbow trout
| Autor: | Daniel Lingwood, L.J. Fisher, James S. Ballantyne, J.W. Callahan |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
inorganic chemicals
Gills Ceramide animal structures Physiology ATPase Biophysics Fresh Water Biology Basement Membrane chemistry.chemical_compound Animals heterocyclic compounds Seawater Na+/K+-ATPase Epithelial polarity chemistry.chemical_classification Sulfoglycosphingolipids Osmolar Concentration Fatty acid Cell Biology Water-Electrolyte Balance biology.organism_classification Adaptation Physiological Trout chemistry Biochemistry Oncorhynchus mykiss cardiovascular system biology.protein Rainbow trout Sodium-Potassium-Exchanging ATPase Fish gill |
| Zdroj: | The Journal of membrane biology. 201(2) |
| ISSN: | 0022-2631 |
| Popis: | We investigated the effect of salinity on the relationship between Na(+)-K(+)-ATPase and sulfogalactosyl ceramide (SGC) in the basolateral membrane of rainbow trout (Oncorhynchus mykiss) gill epithelium. SGC has been implicated as a cofactor in Na(+)-K(+)-ATPase activity, especially in Na(+)-K(+)-ATPase rich tissues. However, whole-tissue studies have questioned this role in the fish gill. We re-examined SGC cofactor function from a gill basolateral membrane perspective. Nine SGC fatty acid species were quantified by tandem mass spectrometry (MS/MS) and related to Na(+)-K(+)-ATPase activity in trout acclimated to freshwater or brackish water (20 ppt). While Na(+)-K(+)-ATPase activity increased, the total concentration and relative proportion of SGC isoforms remained constant between salinities. However, we noted a negative correlation between SGC concentration and Na(+)-K(+)-ATPase activity in fish exposed to brackish water, whereas no correlation existed in fish acclimated to freshwater. Differential Na(+)-K(+)-ATPase/SGC sensitivity is discussed in relation to enzyme isoform switching, the SGC cofactor site model and saltwater adaptation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink