Expression of vascular endothelial growth factor in human brain tumors
| Autor: | Ritsuko Nagashima, Masao Matsutani, Shi Yuan Cheng, Huei-Jen Su Huang, Webster K. Cavenee, Robert M. Nishikawa |
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| Rok vydání: | 1998 |
| Předmět: |
Adenoma
Vascular Endothelial Growth Factor A Pathology medicine.medical_specialty Blotting Western Brain tumor Enzyme-Linked Immunosorbent Assay Endothelial Growth Factors Biology Pituitary neoplasm Pathology and Forensic Medicine Cellular and Molecular Neuroscience chemistry.chemical_compound Pituitary adenoma Meningeal Neoplasms medicine Humans Pituitary Neoplasms Neurons Lymphokines Brain Neoplasms Vascular Endothelial Growth Factors Antibodies Monoclonal medicine.disease Immunohistochemistry Vascular endothelial growth factor Vascular endothelial growth factor A chemistry Astrocytes Germinoma Neurology (clinical) Germ cell tumors Meningioma |
| Zdroj: | Acta Neuropathologica. 96:453-462 |
| ISSN: | 1432-0533 0001-6322 |
| DOI: | 10.1007/s004010050919 |
| Popis: | Compared to normal brain an increased expression of vascular endothelial growth factor (VEGF) has been reported in many types of brain tumors. However, the numbers of samples analyzed and information about the cellular distribution of VEGF have been limited. Here we used novel monoclonal antibodies against VEGF to analyze, using immunohistochemistry, Western blotting and enzyme-linked immunosorbent assay, its expression in 108 human brain tumors that included astrocytic tumors, meningiomas, pituitary adenomas, primary intracranial germ cell tumors and neuronal tumors. The results showed that 37 of 48 astrocytic tumors (77%) and 15 of 19 meningiomas (79%) were immunoreactive for VEGF, consistent with previous reports. However, in contrast to a previous report that analyzed only VEGF mRNA; all of our 15 pituitary adenomas showed specific immunoreactivity for VEGF. We also extended the studies to previously unanalyzed neoplasms: 13 of 15 primary intracranial germ cell tumors (82%), and 7 of 10 neuronal tumors (70%) were immunoreactive for VEGF. Direct protein analysis by Western blotting confirmed the expression of VEGF in those tumors, and showed differential expression of the isoforms of VEGF protein; a pituitary adenoma expressed both VEGF165 and VEGF189 proteins, a central neurocytoma expressed only VEGF165, while an immature teratoma expressed only VEGF189. The data herein show that VEGF is expressed in a wide spectrum of brain tumors and suggest differences among tumor entities in the mechanisms of VEGF up-regulation as well as their employment of distinct VEGF isoforms for neovascularization. |
| Databáze: | OpenAIRE |
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