The Role of Wnt/β-Catenin Signaling Pathway in the Transdifferentiation from Periodontal Ligament Stem Cells to Schwann Cells
| Autor: | Xiaojie Li, Gang Sun, Yan Dong, Dapeng Liao |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Periodontal ligament stem cells Periodontal Ligament Organogenesis Wnt β catenin signaling Biology 03 medical and health sciences 0302 clinical medicine Dogs Animals Myelin Sheath beta Catenin Stem Cells Transdifferentiation Wnt signaling pathway Gene Expression Regulation Developmental Cell Biology Cell biology Wnt Proteins 030104 developmental biology nervous system Cell Transdifferentiation Schwann Cells Signal transduction 030217 neurology & neurosurgery Developmental Biology Biotechnology Signal Transduction |
| Zdroj: | Cellular reprogramming. 19(6) |
| ISSN: | 2152-4998 |
| Popis: | This study was aimed to investigate the role of Wnt/β-catenin signaling pathway in the differentiation from periodontal ligament stem cells (PDLSCs) to Schwann cells (SCs) and the possible mechanisms. FzB was applied to inhibit the Wnt/β-catenin signaling pathway of differentiated PDLSCs (dPDLSCs), and then immunofluorescence, polymerase chain reaction (PCR), and Western blotting analysis were performed to detect SC marker genes and proteins such as S100, glial fibrillary acidic protein (GFAP), and P75NTR. Results showed that when the Wnt/β-catenin signaling pathway was inhibited, the expression of S100, GFAP, and P75NTR protein significantly decreased in dPDLSCs, p 0.05, whereas PCR results showed that expression of SC myelinogenesis-related genes krox-20, Oct-6, P0, and PMP-22 was significantly downregulated at the same time, p 0.05. These results showed that Wnt/β-catenin signaling pathway participated in the differentiation from PDLSCs to SCs, and inhibiting it could inhibit the differentiation process. |
| Databáze: | OpenAIRE |
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