Taxonomic evaluation of species in the Streptomyces hirsutus clade using multi-locus sequence analysis and proposals to reclassify several species in this clade
| Autor: | William W. Metcalf, Ying Huang, David P. Labeda, Xiaoying Rong, Kou San Ju, James R. Doroghazi |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Nucleic Acid Hybridization Sequence Analysis DNA General Medicine Biology Streptomyces pratens Microbiology Streptomyces Bacterial Typing Techniques Streptomyces prasinus Streptomyces bambergiensis 03 medical and health sciences Streptomyces herbaceus 030104 developmental biology Genes Bacterial RNA Ribosomal 16S Streptomyces cyanoalbus Streptomyces prasinopilosus Botany Streptomyces chlorus Phylogeny Ecology Evolution Behavior and Systematics Streptomyces hirsutus |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 66:2444-2450 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | Previous phylogenetic analysis of species of the genus Streptomyces based on 16S rRNA gene sequences resulted in a statistically well-supported clade (100 % bootstrap value) containing eight species that exhibited very similar gross morphology in producing open looped (Retinaculum-Apertum) to spiral (Spira) chains of spiny- to hairysurfaced, dark green spores on their aerial mycelium. The type strains of the species in this clade, specifically Streptomyces bambergiensis, Streptomyces cyanoalbus, Streptomyces emeiensis, Streptomyces hirsutus, Streptomyces prasinopilosus and Streptomyces prasinus, were subjected to multi-locus sequence analysis (MLSA) utilizing partial sequences of the housekeeping genes atpD, gyrB, recA, rpoB and trpB to clarify their taxonomic status. The type strains of several recently described species with similar gross morphology, including Streptomyces chlorus, Streptomyces herbaceus, Streptomyces incanus, Streptomyces pratens and Streptomyces viridis, were also studied along with six unidentified green-spored Streptomyces strains from the ARS Culture Collection. The MLSAs suggest that three of the species under study (S. bambergiensis, S. cyanoalbus and S. emeiensis) represent synonyms of other previously described species (S. prasinus, S. hirsutus and S. prasinopilosus, respectively). These relationships were confirmed through determination of in silico DNA-DNA hybridization estimates based on draft genome sequences. The five recently described species appear to be phylogenetically distinct but the unidentified strains from the ARS Culture Collection could be identified as representatives of S. hirsutus, S. prasinopilosus or S. prasinus. |
| Databáze: | OpenAIRE |
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