Pentagalloyl glucose from Schinus terebinthifolia inhibits growth of carbapenem-resistant Acinetobacter baumannii

Autor: Michelle Lin, Lewis Marquez, Bhuwan K. Chhetri, Anne Marie Sweeney-Jones, Micah Dettweiler, Julia Kubanek, Daniel V. Zurawski, Cassandra L. Quave
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Acinetobacter baumannii
Keratinocytes
0301 basic medicine
Staphylococcus aureus
Magnetic Resonance Spectroscopy
Anacardiaceae
030106 microbiology
Drug Evaluation
Preclinical
lcsh:Medicine
Microbial Sensitivity Tests
Drug resistance
medicine.disease_cause
Antimicrobial resistance
beta-Lactam Resistance
Article
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Antibiotic resistance
Antibiotics
Drug Resistance
Multiple
Bacterial
medicine
Humans
Gallotannin
lcsh:Science
chemistry.chemical_classification
Multidisciplinary
biology
Plant Extracts
Chemistry
Pseudomonas aeruginosa
Antimicrobials
Small molecules
lcsh:R
biology.organism_classification
Antimicrobial
Hydrolyzable Tannins
Anti-Bacterial Agents
030104 developmental biology
Carbapenems
Biofilms
lcsh:Q
Growth inhibition
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-020-72331-w
Popis: The rise of antibiotic resistance has necessitated a search for new antimicrobials with potent activity against multidrug-resistant gram-negative pathogens, such as carbapenem-resistant Acinetobacter baumannii (CRAB). In this study, a library of botanical extracts generated from plants used to treat infections in traditional medicine was screened for growth inhibition of CRAB. A crude extract of Schinus terebinthifolia leaves exhibited 80% inhibition at 256 µg/mL and underwent bioassay-guided fractionation, leading to the isolation of pentagalloyl glucose (PGG), a bioactive gallotannin. PGG inhibited growth of both CRAB and susceptible A. baumannii (MIC 64–256 µg/mL), and also exhibited activity against Pseudomonas aeruginosa (MIC 16 µg/mL) and Staphylococcus aureus (MIC 64 µg/mL). A mammalian cytotoxicity assay with human keratinocytes (HaCaTs) yielded an IC50 for PGG of 256 µg/mL. Mechanistic experiments revealed iron chelation as a possible mode of action for PGG’s activity against CRAB. Passaging assays for resistance did not produce any resistant mutants over a period of 21 days. In conclusion, PGG exhibits antimicrobial activity against CRAB, but due to known pharmacological restrictions in delivery, translation as a therapeutic may be limited to topical applications such as wound rinses and dressings.
Databáze: OpenAIRE
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