HSP70 regulates the function of mitotic centrosomes
| Autor: | Hsiao-Hui Kuo, Tiffany S. Pan, Fu-Chi Yu, Ling-Huei Yih, Chieh-Ting Fang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
NEDD1 Mitosis Centrosome cycle Microtubules Spindle pole body Polymerization 03 medical and health sciences Cellular and Molecular Neuroscience Tubulin Humans HSP70 Heat-Shock Proteins Spindle Poles Molecular Biology Pericentriolar material Microtubule nucleation Pharmacology Centrosome Chemistry Cell Biology Cell biology Spindle checkpoint 030104 developmental biology Molecular Medicine Multipolar spindles Microtubule-Associated Proteins HeLa Cells Protein Binding |
| Zdroj: | Cellular and molecular life sciences : CMLS. 73(20) |
| ISSN: | 1420-9071 |
| Popis: | To establish a functional bipolar mitotic spindle, the centrosome expands and matures, acquiring enhanced activities for microtubule (MT) nucleation and assembly at the onset of mitosis. However, the regulatory mechanisms of centrosome maturation and MT assembly from the matured centrosome are largely unknown. In this study, we showed that heat shock protein (HSP) 70 considerably accumulates at the mitotic centrosome during prometaphase to metaphase and is required for bipolar spindle assembly. Inhibition or depletion of HSP70 impaired the function of mitotic centrosome and disrupted MT nucleation and polymerization from the spindle pole, and may thus result in formation of abnormal mitotic spindles. In addition, HSP70 may associate with NEDD1 and γ-tubulin, two pericentriolar material (PCM) components essential for centrosome maturation and MT nucleation. Loss of HSP70 function disrupted the interaction between NEDD1 and γ-tubulin, and reduced their accumulation at the mitotic centrosome. Our results thus demonstrate a role for HSP70 in regulating centrosome integrity during mitosis, and indicate that HSP70 is required for the maintenance of a functional mitotic centrosome that supports the assembly of a bipolar mitotic spindle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink