Dysregulated expression of proteins associated with ER stress, autophagy and apoptosis in tissues from nonalcoholic fatty liver disease
| Autor: | Nathan J. Cherrington, Soohee Kim, Seungwoo Hwang, Doug Young Ryu, Seung-Woo Lee |
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| Rok vydání: | 2017 |
| Předmět: |
nonalcoholic fatty liver disease
0301 basic medicine autophagy Activating transcription factor digestive system 03 medical and health sciences 0302 clinical medicine Nonalcoholic fatty liver disease Medicine Transcription factor business.industry Endoplasmic reticulum Autophagy Fatty liver apoptosis nutritional and metabolic diseases medicine.disease digestive system diseases 3. Good health 030104 developmental biology Oncology Apoptosis 030220 oncology & carcinogenesis Immunology endoplasmic reticulum stress Unfolded protein response Cancer research business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Seungwoo Lee 1 , Soohee Kim 1 , Seungwoo Hwang 2 , Nathan J. Cherrington 3 and Doug-Young Ryu 1 1 BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Gwanak-gu, Seoul 08826, Republic of Korea 2 Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea 3 College of Pharmacy, University of Arizona, Tucson, AZ 85721, U.S.A. Correspondence to: Doug-Young Ryu, email: dyryu@snu.ac.kr Keywords: nonalcoholic fatty liver disease, endoplasmic reticulum stress, apoptosis, autophagy Received: November 25, 2016 Accepted: June 04, 2017 Published: June 28, 2017 ABSTRACT Nonalcoholic fatty liver disease (NAFLD) is categorized into nonalcoholic fatty liver (NAFL) and nonalcoholic steatohepatitis (NASH) and has emerged as a risk factor for more critical clinical conditions. However, the underlying mechanisms of NAFLD pathogenesis are not fully understood. In this study, expression of proteins associated with endoplasmic reticulum (ER) stress, apoptosis and autophagy were analyzed in normal, NAFL and NASH human livers by western blotting. Levels of some ER stress-transducing transcription factors, including cleaved activating transcription factor 6, were higher in NASH than in the normal tissues. However, the expression of a majority of the ER chaperones and foldases analyzed, including glucose-regulated protein 78 and ER protein 44, was lower in NASH than in the normal tissues. Levels of apoptosis markers, such as cleaved poly (ADP-ribose) polymerase, were also lower in NASH tissues, in which expression of some B-cell lymphoma-2 family proteins was up- or down-regulated compared to the normal tissues. The level of the autophagy substrate p62 was not different in NASH and normal tissues, although some autophagy regulators were up- or down-regulated in the NASH tissues compared to the normal tissues. Levels of most of the proteins analyzed in NAFL tissues were either similar to those in one of the other two types, NASH and normal, or were somewhere in between. Together, these findings suggest that regulation of certain important tissues processes involved in protein quality control and cell survival were broadly compromised in the NAFLD tissues. |
| Databáze: | OpenAIRE |
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