Detection of primary cilia in human glioblastoma
| Autor: | Matthew R. Sarkisian, Brent A. Reynolds, Lan Hoang-Minh, Florian A. Siebzehnrubl, Daniel J. Silver, Susan L. Semple-Rowland, Dorit Siebzehnrubl, Sarah M. Guadiana, Dennis A. Steindler, Loic P. Deleyrolle, Gayathri Srivinasan, Jeffrey K. Harrison |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Cancer Research Axoneme Cell Tumor initiation Biology Article Microtubule Intraflagellar transport Tubulin Glioma Cell Line Tumor medicine Basal body Humans Cilia Aged 80 and over Homeodomain Proteins ADP-Ribosylation Factors Brain Neoplasms Cilium Tumor Suppressor Proteins Zinc Finger E-box-Binding Homeobox 1 Middle Aged medicine.disease Immunohistochemistry Basal Bodies Cell biology Microscopy Electron medicine.anatomical_structure Ki-67 Antigen Neurology Oncology Cell culture Neurology (clinical) Glioblastoma Transcription Factors |
| Popis: | Glioblastoma (GBM) is the most common malignant adult brain tumor and carries a poor prognosis due to primary and acquired resistance. While many cellular features of GBM have been documented, it is unclear if cells within these tumors extend a primary cilium, an organelle whose associated signaling pathways may regulate proliferation, migration, and survival of neural precursor and tumor cells. Using immunohistochemical and electron microscopy (EM) techniques, we screened human GBM tumor biopsies and primary cell lines for cilia. Immunocytochemical staining of five primary GBM cell lines revealed that between 8 and 25 % of the cells in each line possessed gamma tubulin-positive basal bodies from which extended acetylated, alpha-tubulin-positive axonemes. EM analyses confirmed the presence of cilia at the cell surface and revealed that their axonemes contained organized networks of microtubules, a structural feature consistent with our detection of IFT88 and Arl13b, two trafficked cilia proteins, along the lengths of the axonemes. Notably, cilia were detected in each of 23 tumor biopsies (22 primary and 1 recurrent) examined. These cilia were distributed across the tumor landscape including regions proximal to the vasculature and within necrotic areas. Moreover, ciliated cells within these tumors co-stained with Ki67, a marker for actively dividing cells, and ZEB1, a transcription factor that is upregulated in GBM and linked to tumor initiation, invasion, and chemoresistance. Collectively, our data show that subpopulations of cells within human GBM tumors are ciliated. In view of mounting evidence supporting roles of primary cilia in tumor initiation and propagation, it is likely that further study of the effects of cilia on GBM tumor cell function will improve our understanding of GBM pathogenesis and may provide new directions for GBM treatment strategies. |
| Databáze: | OpenAIRE |
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