A Protein Associated with Toll-Like Receptor 4 (PRAT4A) Regulates Cell Surface Expression of TLR4
| Autor: | Makiko Kobayashi, Yasushi Ishihama, Kazunori Konno, Taketoshi Mizutani, Koichiro Takahashi, Yasutaka Wakabayashi, Kensuke Miyake, Natsuko Tanimura, Sachiko Akashi-Takamura, Yoshiya Oda, Kiyoshi Takatsu, Shin-ichiroh Saitoh, Satoshi Takaki, Hideo Iba, Takashi Ishii, Taku Kouro |
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| Rok vydání: | 2006 |
| Předmět: |
Receptor complex
Glycosylation Molecular Sequence Data Immunology Cell Lymphocyte Antigen 96 Down-Regulation Biology Cell Line Mice medicine Animals Humans Immunology and Allergy Amino Acid Sequence Cloning Molecular Peptide sequence Mice Knockout Gene knockdown Toll-like receptor Membrane Glycoproteins Cell Membrane Dendritic Cells Molecular biology Cell biology Mice Inbred C57BL Toll-Like Receptor 4 TLR2 medicine.anatomical_structure Cell culture TLR4 lipids (amino acids peptides and proteins) Carrier Proteins Molecular Chaperones |
| Zdroj: | The Journal of Immunology. 177:1772-1779 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | TLRs recognize microbial products. Their subcellular distribution is optimized for microbial recognition. Little is known, however, about mechanisms regulating the subcellular distribution of TLRs. LPS is recognized by the receptor complex consisting of TLR4 and MD-2. Although MD-2, a coreceptor for TLR4, enhances cell surface expression of TLR4, an additional mechanism regulating TLR4 distribution has been suggested. We show here that PRAT4A, a novel protein associated with TLR4, regulates cell surface expression of TLR4. PRAT4A is associated with the immature form of TLR4 but not with MD-2 or TLR2. PRAT4A knockdown abolished LPS responsiveness in a cell line expressing TLR4/MD-2, probably due to the lack of cell surface TLR4. PRAT4A knockdown down-regulated cell surface TLR4/MD-2 on dendritic cells. These results demonstrate a novel mechanism regulating TLR4/MD-2 expression on the cell surface. |
| Databáze: | OpenAIRE |
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