Fabrication of a Novel Cell Culture System Using DNA-Grafted Substrates and DNase
| Autor: | Yasutaka Matsuo, Kohji Nakazawa, Kuniharu Ijiro, Hideyuki Mitomo, Kenichi Niikura, Asumi Eguchi, Yasunobu Suzuki |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
010407 polymers Cell Survival Cell Induced Pluripotent Stem Cells Biomedical Engineering Cell Culture Techniques Pharmaceutical Science Medicine (miscellaneous) Bioengineering 01 natural sciences Substrate Specificity 03 medical and health sciences Hydrolysis chemistry.chemical_compound Mice medicine Extracellular Cell Adhesion Animals Humans General Materials Science Cell Proliferation Fluorescent Dyes chemistry.chemical_classification Deoxyribonucleases Feeder Cells Water DNA Trypsin 0104 chemical sciences Cell biology 030104 developmental biology Enzyme medicine.anatomical_structure Membrane chemistry Cell culture NIH 3T3 Cells medicine.drug HeLa Cells |
| Zdroj: | Journal of biomedical nanotechnology. 12(2) |
| ISSN: | 1550-7033 |
| Popis: | In conventional cell culture systems, trypsin is generally used for cell harvesting. However, trypsin damages the cells due to the nonselective degradation of proteins on the cell surface. This is a critical issue for cell culture systems. Therefore, an alternative cell culture system with the lowest possible impact on cells is desired. In this paper, we have focused on DNA as a sacrificial layer and DNase as an alternate enzyme instead of trypsin. DNase ought not to result in damage to or stress on cells as it only hydrolyzes DNAs while the plasma membrane and extracellular matrices are basically composed of lipids, proteins, and glycosides. Therefore, we fabricated DNA-grafted substrates as cell culture dishes and evaluated this novel cell culture system. As a result, we were able to culture several types of mammalian cells on the DNA-grafted substrates, with the cells harvested using DNase with only little damage to the cells. This cell culture system could provide a breakthrough in cell culturing technology. |
| Databáze: | OpenAIRE |
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