Sensitization and Elicitation of an Allergic Reaction to Wheat Gliadins in Mice
| Autor: | M. Leroy, Karine Adel-Patient, J.-M. Wal, Fany Blanc, S. Ah-Leung, Sandra Denery-Papini, Olivier Tranquet, Marie Bodinier |
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| Přispěvatelé: | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Unité de Recherche Immuno-Allergie Alimentaire |
| Rok vydání: | 2009 |
| Předmět: |
Allergy
Immunoglobulin E Gliadin Mice 0302 clinical medicine Antibody Specificity [SDV.IDA]Life Sciences [q-bio]/Food engineering Child Triticum Sensitization Mice Inbred BALB C Mice Inbred C3H 0303 health sciences biology Chemistry INDUCTION food and beverages MOUSE MODEL 3. Good health ALLERGY medicine.anatomical_structure Plant protein Cytokines Female IgE Antibody General Agricultural and Biological Sciences Adult IgG WHEAT Wheat flour 03 medical and health sciences Th2 Cells GLIADINS Hypersensitivity Splenocyte medicine Animals Humans 030304 developmental biology nutritional and metabolic diseases General Chemistry medicine.disease digestive system diseases Eosinophils Disease Models Animal 030228 respiratory system Immunoglobulin G Immunology biology.protein Immunization |
| Zdroj: | Journal of Agricultural and Food Chemistry Journal of Agricultural and Food Chemistry, American Chemical Society, 2009, 57 (4), pp.1219-1225. ⟨10.1021/jf802898u⟩ |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf802898u |
| Popis: | International audience; We developed a mouse model of allergy to wheat flour gliadins, a protein fraction containing major wheat allergens. We compared the antibody responses (i.e., specific IgE and IgG1) and the profiles of cytokines secreted by reactivated splenocytes induced after intraperitoneal injections of gliadins in three strains of mice, namely, Balb/cJ, B10.A, and C3H/HeJ. The intensities of the allergic reactions elicited by intranasal challenge were also compared. Both the sensitization and elicitation were the highest in Balb/cJ mice, whereas weak or no reaction was observed in the others strains. Interestingly, the specificity of the mouse IgE against the different gliadins (i.e., alpha-, beta-, gamma-, omega 1,2-, and omega 5-gliadin) was similar to that observed in children allergic to wheat flour. Balb/cJ mice may thus provide a relevant model for the study of sensitization and elicitation by wheat gliadins and for improving our understanding of the specific role and mechanisms of action of the different classes of gliadins. |
| Databáze: | OpenAIRE |
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