Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with real-Time PCR on frass
| Autor: | Eleonora Barra, L. Stefani, Francesco Nugnes, Paola Spigno, Antonio P. Garonna, Linda Bartolini, Domenico Rizzo, Raffaele Griffo, Elisabetta Rossi, Andrea Taddei, Daniele Da Lio, Lucia Cozzolino |
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| Přispěvatelé: | Rizzo, D., Taddei, A., Da Lio, D., Nugnes, F., Barra, E., Stefani, L., Bartolini, L., Griffo, R. V., Spigno, P., Cozzolino, L., Rossi, E., Garonna, A. P. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Integrated pest management non-invasive diagnostic tool Geography Planning and Development TJ807-830 Zoology Management Monitoring Policy and Law Biology TD194-195 01 natural sciences Renewable energy sources law.invention 03 medical and health sciences Prunus law Quarantine media_common.cataloged_instance GE1-350 European union 030304 developmental biology Phytosanitary certification media_common xylophagous insect 0303 health sciences Environmental effects of industries and plants Renewable Energy Sustainability and the Environment quarantine pest Frass Frass DNA Non-invasive diagnostic tool Phytosanitary survey Quarantine pest Xylophagous insect fungi food and beverages Environmental sciences 010602 entomology phytosanitary survey frass DNA PEST analysis Longhorn beetle |
| Zdroj: | Sustainability (Basel) 12 (2020). doi:10.3390/su12156041 info:cnr-pdr/source/autori:Rizzo D., Taddei A., Da Lio D., Nugnes F., Barra E., Stefani L., Bartolini L., Griffo R.V., Spigno P., Cozzolino L., Rossi E., Garonna A.P./titolo:Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with real-Time PCR on frass/doi:10.3390%2Fsu12156041/rivista:Sustainability (Basel)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:12 Sustainability Volume 12 Issue 15 Sustainability, Vol 12, Iss 6041, p 6041 (2020) |
| Popis: | Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae), the red-necked longhorn beetle is native to eastern Asia, where it is an important wood-borer of fruit and ornamental species of the genus Prunus. A. bungii is a quarantine pest in the European Union, following its accidental introduction and establishment in Germany and Italy, and is currently included in the list of priority pests. To confirm its infestations in outbreak areas, adult or larval specimens are needed to perform morphological or molecular analyses. The presence of A. bungii larvae inside the attacked trees makes the collection of specimens particularly difficult. Thus, we present two diagnostic protocols based on frass analysis with real-time PCR (probe and SYBR Green). The results obtained show that a non-invasive approach for detecting the presence of this harmful invasive pest can be a reliable and accurate alternative diagnostic tool in phytosanitary surveys, as well as to outline a sustainable pest management strategy. |
| Databáze: | OpenAIRE |
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