Dynamic gene expression is required for anterior regionalization in a spider
| Autor: | Wim G.M. Damen, Natália Martins Feitosa, Matthias Pechmann, Evelyn E. Schwager, Alistair P. McGregor |
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| Rok vydání: | 2009 |
| Předmět: |
animal structures
Molecular Sequence Data Gene expression Basic Helix-Loop-Helix Transcription Factors In Situ Nick-End Labeling Achaearanea Animals Drosophila Proteins Hedgehog Proteins Hedgehog In Situ Hybridization Genetics Parasteatoda tepidariorum Multidisciplinary biology Gene Expression Regulation Developmental Drosophila embryogenesis Spiders Embryo Biological Sciences biology.organism_classification Cell biology Repressor Proteins embryonic structures Insect Proteins RNA Interference Blastoderm Morphogen |
| Zdroj: | Proceedings of the National Academy of Sciences. 106:1468-1472 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Patterning of a multicellular embryo requires precise spatiotemporal control of gene expression during development. The gradient of the morphogen bicoid regulates anterior regionalization in the syncytial blastoderm of Drosophila . However many arthropod embryos develop from a cellular blastoderm that does not allow the formation of transcription factor gradients. Here we show that correct anterior development of the cellularized embryo of the spider Achaearanea tepidariorum requires an anterior-to-posterior wave of dynamic gene expression for positioning the stripes of hairy , hedgehog , and orthodenticle expression. Surprisingly, this dynamic repositioning of the expression of these segmentation genes is blocked in orthodenticle pRNAi embryos and no anterior structures are specified in those embryos. Our data suggest that dynamic gene expression across a field of cells is required for anterior regionalization in spiders and provides an explanation for the problem of how positional values for anterior segmentation genes are specified via a morphogen-independent mechanism across a field of cells. |
| Databáze: | OpenAIRE |
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