An Intact Brachyury Function Is Necessary to Prevent Spurious Axial Development in Xenopus laevis

Autor: Andrés E. Carrasco, Cecilia Elena Aguirre, Sabrina Murgan, Silvia L. López
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Fetal Proteins
Embryology
Xenopus
Developmental Signaling
lcsh:Medicine
Xenopus Proteins
purl.org/becyt/ford/1 [https]
Xenopus laevis
Molecular Cell Biology
Morphogenesis
Basic Helix-Loop-Helix Transcription Factors
lcsh:Science
In Situ Hybridization
Multidisciplinary
Biología del Desarrollo
Animal Models
Anatomy
Zoología
Ornitología
Entomología
Etología
Signaling in Selected Disciplines
Marginal zone
Cell biology
NOTCH
medicine.anatomical_structure
BRACHYURY
CIENCIAS NATURALES Y EXACTAS
Research Article
Signal Transduction
Brachyury
Mesoderm
Biology
Ciencias Biológicas
Model Organisms
Notochord
medicine
Animals
Birth Defects
purl.org/becyt/ford/1.6 [https]
Transcription factor
Body Patterning
lcsh:R
Molecular Development
Body Plan Organization
biology.organism_classification
Signaling
Gastrulation
VERTEBRATE DEVELOPMENT
lcsh:Q
Ectopic expression
DORSAL MIDLINE
T-Box Domain Proteins
Developmental Biology
Zdroj: PLoS ONE, Vol 8, Iss 1, p e54777 (2013)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLoS ONE
DOI: 10.1371/journal.pone.0054777
Popis: We have previously shown that the member of the HES family hairy2 induces the ectopic expression of dorsal markers when it is overexpressed in the ventral side of Xenopus embryos. Intriguingly, hairy2 represses the mesoderm transcription factor brachyury (bra) throughout its domain in the marginal zone. Here we show that in early gastrula, bra and hairy2 are expressed in complementary domains. Overexpression of bra repressed hairy2. Interference of bra function with a dominant-negative construct expanded the hairy2 domain and, like hairy2 overexpression, promoted ectopic expression of dorsal axial markers in the ventral side and induced secondary axes without head and notochord. Hairy2 depletion rescued the ectopic dorsal development induced by interference of bra function. We concluded that an intact bra function is necessary to exclude hairy2 expression from the non-organiser field, to impede the ectopic specification of dorsal axial fates and the appearance of incomplete secondary axes. This evidence supports a previously unrecognised role for bra in maintaining the dorsal fates inhibited in the ventral marginal zone, preventing the appearance of trunk duplications. Fil: Aguirre, Cecilia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina Fil: Murgan, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina Fil: Carrasco, Andres Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina Fil: Lopez, Silvia Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina
Databáze: OpenAIRE
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