The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia
| Autor: | Cleary, David, Morris, Denise, Anderson, Rebecca, Jones, Jessica, Alattraqchi, Ahmed Ghazi, Rahman, Nor Iza, Ismail, Salwani, Razali, Mohamad, Amin, Rahmah Mohd, Aziz, Aniza Abd, Esa, Nor Kamarruzaman, Amiruddin, Salman, Chew, Ching Hoong, Amat Simin, Mohamad Hafis, Abdullah, Ramle, Yeo, Chew Chieng, Clarke, Stuart |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Respiratory System Drug resistance medicine.disease_cause Applied Microbiology and Biotechnology Microbial ecology RNA Ribosomal 16S Prevalence Prevotella Cluster Analysis Young adult Child biology Streptococcus Microbiota QR100-130 Middle Aged Anti-Bacterial Agents Streptococcus pneumoniae Child Preschool Female Neisseria Pathogens Nasal Cavity Biotechnology Adult Adolescent Aggregatibacter 030106 microbiology Veillonella Serogroup Microbiology Policy and public health in microbiology Indigenous Article 03 medical and health sciences Young Adult Antibiotic resistance Drug Resistance Bacterial Haemophilus medicine Humans Microbiome Indigenous Peoples Moraxella Aged Mouth Bacteria Malaysia biology.organism_classification 030104 developmental biology Carriage Demography |
| Zdroj: | NPJ Biofilms and Microbiomes npj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-11 (2021) |
| Popis: | BackgroundMicrobiome research has focused on populations that are predominantly of European descent, and from narrow demographics that do not capture the socio-economic and lifestyle differences which impact human health. This limits our understanding of human-host microbiota interactions in their broadest sense. Here we examined the airway microbiology of the Orang Asli, the indigenous peoples of Malaysia. In addition to exploring the carriage and antimicrobial resistance of important respiratory pathobionts, we also present the first investigation of the nasal microbiomes of these indigenous peoples, in addition to their oral microbiomes.ResultsA total of 130 participants were recruited to the study from Kampung Sungai Pergam and Kampung Berua, both sites in the north-eastern state of Terengganu in Peninsular Malaysia. High levels ofStaphylococcus aureuscarriage were observed, particularly in the 18-65 age group (n=17/36; 47.2% 95%CI: 30.9-63.5). The highest carriage of pneumococci was in the p= 0.001). Hierarchical clustering of Bray-Curtis dissimilarity scores revealed six microbiome types. The largest cluster (n=28; 35.4%) had a marked abundance ofCorynebacterium.Others comprisedCorynebacteriumwithDolosigranulum, two clusters were definable by the presence ofMoraxella, one with and the other withoutHaemophilus, a small grouping ofDelftia/ Ochrobactumprofiles and one withStreptococcus. NoStaphylococcusprofiles were observed. In the oral microbiomesStreptococcus, NeisseriaandHaemophiluswere dominant. Lower levels ofPrevotella, Rothia, Porphyromonas, VeillonellaandAggregatibacterwere also among the eight most observed genera.ConclusionsWe present the first study of Orang Asli airway microbiomes and pathobiont microbiology. Key findings include the prevalence of pneumococcal serotypes that would be covered by pneumococcal conjugate vaccines if introduced into a Malaysian national immunisation schedule, and the high level ofS. aureuscarriage. The dominance ofCorynebacteriumin the airway microbiomes is particularly intriguing given its’ consideration as a potentially protective commensal with respect to acute infection and respiratory health. |
| Databáze: | OpenAIRE |
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