Ribozyme mediated cleavage of acute phase serum amyloid A (A-SAA) mRNA in vitro
| Autor: | Diana M. Steel, Derval J. Gaughan, Alexander S. Whitehead |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Hammerhead ribozyme
animal diseases Molecular Sequence Data Biophysics Acute phase response Inflammation Biochemistry Substrate Specificity Structural Biology hemic and lymphatic diseases Genetics medicine RNA Catalytic Serum amyloid A RNA Messenger Overproduction Molecular Biology DNA Primers Messenger RNA Serum Amyloid A Protein Binding Sites biology Base Sequence Ribozyme Acute-phase protein Cell Biology biology.organism_classification Molecular biology In vitro stomatognathic diseases biology.protein Nucleic Acid Conformation medicine.symptom Acute-Phase Proteins |
| Zdroj: | FEBS letters. 374(2) |
| ISSN: | 0014-5793 |
| Popis: | The 1000-fold induction of acute phase serum amyloid A (A-SAA) in the liver during inflammation indicates that this protein plays an important, though ill-defined, role in host defence. Paradoxically, prolonged overproduction of A-SAA is a causative factor in secondary amyloidosis and possibly other diseases such as atherosclerosis; the ability to down-regulate A-SAA synthesis is therefore of considerable clinical importance. We have successfully generated anti-SAA hammerhead ribozymes and we report that they are capable of cleaving A-SAA mRNA in vitro. |
| Databáze: | OpenAIRE |
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