Application of Sample6 DETECT™ HT/L Kit for the Detection of Listeria in Mixed Leafy Greens
| Autor: | Kristina Tenney, Tam Mai, Brandon Selover, Mansour Samadpour, Elba Veronica Arias-Rios, Joseph Lorr, Lourdes M Nadala, Seana K Davidson, Ruth Marie Cantera |
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| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Chromatography biology Listeria Chemistry Early detection biology.organism_classification Listeria monocytogenes Culture Media Analytical Chemistry Food and drug administration Method comparison Food Microbiology Environmental Chemistry Bacteriophages Agronomy and Crop Science Leafy Plate reader Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 103:156-160 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | Background: Early and accurate detection of Listeria in foods is vital. Current methods require 24 h enrichment for detection. Objective: This study aimed to demonstrate enhanced early detection of Listeria in mixed leafy greens using Sample6 DETECT™ HT/L, a phage-based detection system. Methods: A method comparison between the reference method (U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 10) for the detection of Listeria spp. and the Sample6 DETECT HT/L using a new proprietary R2 Medium was performed in mixed leafy greens. Results: Using the R2 Medium enrichment with Sample6 DETECT HT/L, detection of L. innocua was possible at 12 h (with a centrifugation step), and L. monocytogenes was detected by 18 h, with equivalent performance as the 24 h enrichments using the reference method detection. The Sample6 DETECT HT/L gave an equivalent performance for L. innocua at 15 h as the reference method at 24 h. Detection was accomplished by the addition of reagents in the kit, following the package insert, and reading results in a detection chamber using a 96-well plate reader that detects a fluorescent signal. Conclusions: Results indicate the new R2 Medium and Sample6 DETECT HT/L allowed for earlier detection of Listeria spp. in mixed leafy greens. Highlights: Sample6 DETECT HT/L with the new R2 Medium allowed the early detection of Listeria spp. and may be applied in other food matrices and environmental samples. |
| Databáze: | OpenAIRE |
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