Innate immune functions of avian intestinal epithelial cells: Response to bacterial stimuli and localization of responding cells in the developing avian digestive tract

Autor: Aharon Friedman, Enav Bar Shira
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Lipopolysaccharides
Salmonella typhimurium
0301 basic medicine
Embryology
lcsh:Medicine
Chick Embryo
Epithelium
Poultry
0302 clinical medicine
Intestinal mucosa
Animal Cells
Medicine and Health Sciences
Gamefowl
Intestinal Mucosa
lcsh:Science
Cells
Cultured
Staining
Mammals
Multidisciplinary
Antimicrobials
Cell Staining
Eukaryota
Drugs
Intestinal epithelium
Cell biology
medicine.anatomical_structure
Vertebrates
Anatomy
Cellular Types
Bacillus subtilis
Research Article
Staphylococcus aureus
Secretory component
Biology
Research and Analysis Methods
Microbiology
Avian Proteins
Birds
03 medical and health sciences
Immune system
Microbial Control
Escherichia coli
medicine
Animals
Pharmacology
Goblet cell
Innate immune system
Embryos
lcsh:R
Organisms
Biology and Life Sciences
Epithelial Cells
Cell Biology
Avidin
Immunity
Innate
Secretory Component
Gastrointestinal Tract
Biological Tissue
030104 developmental biology
Specimen Preparation and Treatment
Fowl
Amniotes
Paneth cell
Antibacterials
Muramidase
lcsh:Q
Polymeric immunoglobulin receptor
Chickens
Digestive System
Developmental Biology
030215 immunology
Zdroj: PLoS ONE, Vol 13, Iss 7, p e0200393 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Intestinal epithelial cells are multi-tasked cells that participate in digestion and absorption as well as in protection of the digestive tract. While information on the physiology and immune functions of intestinal epithelial cells in mammals is abundant, little is known of their immune function in birds and other species. Our main objectives were to study the development of anti-bacterial innate immune functions in the rapidly developing gut of the pre- and post-hatch chick and to determine the functional diversity of epithelial cells. After establishing primary intestinal epithelial cell cultures, we demonstrated their capacity to uptake and process bacteria. The response to bacterial products, LPS and LTA, induced expression of pro-inflammatory cytokine genes (IL-6, IL-18) as well as the expression of the acute phase proteins avidin, lysozyme and the secretory component derived from the polymeric immunoglobulin receptor. These proteins were then localized in gut sections, and the goblet cell was shown to store avidin, lysozyme as well as secretory component. Lysozyme staining was also located in a novel rod-shaped intestinal cell, situated at different loci along the villus, thus deviating from the classical Paneth cell in the mammal, that is restricted to crypts. Thus, in the chicken, the intestinal epithelium, and particularly goblet cells, are committed to innate immune protection. The unique role of the goblet cell in chicken intestinal immunity, as well as the unique distribution of lysozyme-positive cells highlight alternative solutions of gut protection in the bird.
Databáze: OpenAIRE
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