Human serum γ-globulin binds copper cations
| Autor: | S. B. Cheknyov, U. A. Vorobyova, E. E. Babaeva, M. S. Zharkova |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Protein Conformation
Inorganic chemistry Ultrafiltration chemistry.chemical_element General Biochemistry Genetics and Molecular Biology Metal chemistry.chemical_compound Protein structure Cations Spectrophotometry medicine Humans Molecule Binding site Sodium diethyldithiocarbamate Binding Sites medicine.diagnostic_test General Medicine Copper Ultrafiltration (renal) chemistry visual_art visual_art.visual_art_medium Spectrophotometry Ultraviolet gamma-Globulins |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 141:53-56 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-006-0092-5 |
| Popis: | Binding of copper cations to human serum gamma-globulin was studied using molecular ultrafiltration. The content of free metal in the filtrate was evaluated by the reaction with sodium diethyldithiocarbamate. Conformation characteristics of the protein were evaluated by UV spectrophotometry. gamma-Globulin molecule has several copper-binding sites differing by binding constants and filled one-by-one as the content of bound metal increased. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink