Increased Activity of Interleukin-6 but not Tumor Necrosis Factor-α in Lung Lavage of Premature Infants is Associated with the Development of Bronchopulmonaiy Dysplasia
| Autor: | Vicki Taciak, J. E. Ensor, Rose M. Viscardi, Alakananda Bagchi, Jeffrey D. Hasday, Kimberley A Mccrea |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_treatment Inflammation Pregnancy Risk Factors mental disorders medicine Humans Bronchopulmonary Dysplasia Respiratory Distress Syndrome Newborn Lung medicine.diagnostic_test Respiratory distress Interleukin-6 Tumor Necrosis Factor-alpha business.industry Respiratory disease Age Factors Infant Newborn medicine.disease Bronchoalveolar lavage medicine.anatomical_structure Cytokine Bronchopulmonary dysplasia Pediatrics Perinatology and Child Health Immunology Female Tumor necrosis factor alpha medicine.symptom business Bronchoalveolar Lavage Fluid Biomarkers Infant Premature |
| Zdroj: | Pediatric Research. 36:244-252 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-199408000-00017 |
| Popis: | Although considerable evidence suggests that bronchopulmonary dysplasia (BPD) is the result of prolonged inflammation and impaired healing of the immature lung, the mediators that regulate inflammation in neonatal lung injury have not been completely elucidated. We examined whether the cytokines IL-6 and tumor necrosis factor-α (TNF) interact to modulate a cascade of cell-cell signaling events involved in inflammation contributing to the development of BPD. To determine the relative activities of these cytokines in neonatal lung injury, lung lavage samples were serially obtained from 1 to 28 d from 11 infants with self-limited respiratory distress syndrome (RDS), 19 infants with evolving BPD, and 10 control infants ventilated for nonpulmonary reasons. On the first day of life, there were no differences in antigenic IL-6 concentrations in lavage fluids among the BPD, RDS, and control groups, but IL-6 activity determined by the 7TD1 proliferation assay was 15-fold and 6.6-fold higher in lung lavage of infants who developed BPD compared with activities in lavage from control and RDS infants, respectively (control, 49.4 ± 17.6; RDS, 117.3 ± 59.6; BPD, 779.5 ± 212.6 ± 103 hybridoma units/L, mean ± SEM, p = 0.02). This suggests that pathways for inactivating or inhibiting IL-6 that may be present in the lungs of RDS and control infants may be deficient in BPD infants. IL-6 activity remained elevated in lavage of BPD infants for the first 2 wk and declined to low levels by d 28. There were no differences among groups on the first day of life for TNF antigen concentration or TNF activity determined by the L929 bioassay. Detectable but low TNF activity was found in BPD samples, with peak activity found in d-14 samples. Differences in complex interactions among these and other cytokines with their receptors and inhibitors may predispose some infants with RDS to develop BPD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|