Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila
| Autor: | Anette Schneemann, Catherine Dostert, Jules A. Hoffmann, Delphine Galiana-Arnoux, Jean-Luc Imler |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Ribonuclease III
Sindbis virus viruses Immunology Virus Replication Virus Animals Genetically Modified Viral Proteins RNA interference Animals Drosophila Proteins RNA Viruses Immunology and Allergy Nodaviridae biology fungi RNA biology.organism_classification Virology Drosophila melanogaster Viral replication Mutation biology.protein RNA Viral RNA Interference Drosophila C virus RNA Helicases Dicer |
| Zdroj: | Nature Immunology. 7:590-597 |
| ISSN: | 1529-2916 1529-2908 |
| DOI: | 10.1038/ni1335 |
| Popis: | The fruit fly Drosophila melanogaster is a model system for studying innate immunity, including antiviral host defense. Infection with drosophila C virus triggers a transcriptional response that is dependent in part on the Jak kinase Hopscotch. Here we show that successful infection and killing of drosophila with the insect nodavirus flock house virus was strictly dependent on expression of the viral protein B2, a potent inhibitor of processing of double-stranded RNA mediated by the essential RNA interference factor Dicer. Conversely, flies with a loss-of-function mutation in the gene encoding Dicer-2 (Dcr-2) showed enhanced susceptibility to infection by flock house virus, drosophila C virus and Sindbis virus, members of three different families of RNA viruses. These data demonstrate the importance of RNA interference for controlling virus replication in vivo and establish Dcr-2 as a host susceptibility locus for virus infections. |
| Databáze: | OpenAIRE |
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