Huckebein is part of a combinatorial repression code in the anterior blastoderm
| Autor: | Lilian Ponce de Barros, Ariane Machado-Lima, Luciano Antonio Digiampietri, Luiz Paulo Andrioli |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pair-rule stripes
Embryo Nonmammalian Body Patterning Fushi Tarazu Transcription Factors Repressor Biology Mechanisms of gene repression Segmentation Transcriptional regulation Basic Helix-Loop-Helix Transcription Factors Image Processing Computer-Assisted Animals Drosophila Proteins Blastoderm Gap genes Molecular Biology Psychological repression In Situ Hybridization Gap gene Homeodomain Proteins Genetics Binding Sites fungi Runt Computational Biology Nuclear Proteins food and beverages Cell Biology Cell biology DNA-Binding Proteins Repressor Proteins DROSOPHILA Segment polarity gene embryonic structures Drosophila Drosophila Protein Transcription Factors Developmental Biology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2011.10.016 |
| Popis: | The hierarchy of the segmentation cascade responsible for establishing the Drosophila body plan is composed by gap, pair-rule and segment polarity genes. However, no pair-rule stripes are formed in the anterior regions of the embryo. This lack of stripe formation, as well as other evidence from the literature that is further investigated here, led us to the hypothesis that anterior gap genes might be involved in a combinatorial mechanism responsible for repressing the cis-regulatory modules (CRMs) of hairy (h), even-skipped (eve), runt (run), and fushi-tarazu (ftz) anterior-most stripes. In this study, we investigated huckebein (hkb), which has a gap expression domain at the anterior tip of the embryo. Using genetic methods we were able to detect deviations from the wild-type patterns of the anterior-most pair-rule stripes in different genetic backgrounds, which were consistent with Hkb-mediated repression. Moreover, we developed an image processing tool that, for the most part, confirmed our assumptions. Using an hkb misexpression system, we further detected specific repression on anterior stripes. Furthermore, bioinformatics analysis predicted an increased significance of binding site clusters in the CRMs of h 1, eve 1, run 1 and ftz 1when Hkb was incorporated in the analysis, indicating that Hkb plays a direct role in these CRMs. We further discuss that Hkb and Slp1, which is the other previously identified common repressor of anterior stripes, might participate in a combinatorial repression mechanism controlling stripe CRMs in the anterior parts of the embryo and define the borders of these anterior stripes. |
| Databáze: | OpenAIRE |
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