The breast cancer resistance protein transporter ABCG2 is expressed in the human kidney proximal tubule apical membrane
| Autor: | Suzanne Heemskerk, Rachel Sayer, Colin D.A. Brown, Miriam Huls, Amy S. Windass, Rosalinde Masereeuw, Frans G. M. Russel, J. J. M. W. van den Heuvel |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
animal structures Abcg2 Membrane transport and intracellular motility [NCMLS 5] cell and transport physiology ATP-binding cassette transporter Synthetic Organic Chemistry ABCC4 Polymerase Chain Reaction Kidney Tubules Proximal Metabolism transport and motion [NCMLS 2] Mice Internal medicine medicine ATP Binding Cassette Transporter Subfamily G Member 2 Animals Humans RNA Messenger Renal disorder [IGMD 9] immunostaining Kidney biology Multidrug resistance-associated protein 2 Cell Membrane Transporter Apical membrane Immunohistochemistry Molecular biology Multidrug Resistance-Associated Protein 2 Neoplasm Proteins Rats Renal disorders [UMCN 5.4] medicine.anatomical_structure Endocrinology drug excretion Nephrology biology.protein ATP-Binding Cassette Transporters ABC transporter Efflux |
| Zdroj: | Kidney International. Supplement, 73, 220-5 Kidney International. Supplement, 73, 2, pp. 220-5 |
| ISSN: | 0085-2538 2157-1724 |
| DOI: | 10.1038/sj.ki.5002645 |
| Popis: | Contains fulltext : 70782.pdf (Publisher’s version ) (Closed access) The Breast Cancer Resistance Protein (BCRP/ABCG2) is a transporter restricting absorption and enhancing excretion of many compounds including anticancer drugs. This transporter is highly expressed in many tissues; however, in human kidney, only the mRNA was found in contrast to the mouse kidney, where the transporter is abundant. In bcrp/abcg2((-/-)) mice, the expression of two sterol transporter genes, abcg5 and abcg8, was strongly increased in the kidney, perhaps as a compensatory mechanism to upregulate efflux. We found using immunohistochemical analysis clear localization of BCRP/ABCG2 to the proximal tubule brush border membrane of the human kidney comparable to that of other ABC transporters such as P-glycoprotein/ABCB1, MRP2/ABCC2, and MRP4/ABCC4. Hoechst 33342 dye efflux from primary human proximal tubule cells was significantly reduced by the BCRP/ABCG2 inhibitors fumitremorgin C and nelfinavir. Our study shows that in addition to other apical ABC transporters, BCRP/ABCG2 may be important in renal drug excretion. |
| Databáze: | OpenAIRE |
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