Genome-wide analysis of DNA methylation in tongue squamous cell carcinoma
| Autor: | Hanjiang Wu, Lei Shi, Xiang-Ling Feng, Tian-You Ling, Chao-Jian Gong, Zhi-Jing He, Sheng Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
rho GTP-Binding Proteins
Cancer Research Carcinogenesis Proteinase Inhibitory Proteins Secretory bcl-X Protein Biology medicine.disease_cause Antigens CD Antigens Neoplasm medicine Humans RNA Messenger Methylated DNA immunoprecipitation Gene Microarray analysis techniques Calcium-Binding Proteins General Medicine Methylation DNA Methylation Neoplasm Proteins Tongue Neoplasms Up-Regulation Reverse transcription polymerase chain reaction Core Binding Factor Alpha 3 Subunit Oncology CpG site DNA methylation Carcinoma Squamous Cell Disease Progression Cancer research CpG Islands Cell Adhesion Molecules Genome-Wide Association Study |
| Zdroj: | Oncology Reports. 29:1819-1826 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2013.2309 |
| Popis: | Tongue squamous cell carcinoma (TSCC) is one of the most common types of oral cancer; however, its molecular mechanisms remain unclear. In this study, methylated DNA immunoprecipitation (MeDIP) coupled with methylation microarray analysis was performed to screen for aberrantly methylated genes in adjacent normal control and TSCC tissues from 9 patients. Roche NimbleGen Human DNA Methylation 385K Promoter Plus CpG Island Arrays were used to detect 28,226 CpG sites. A total of 1,269 hypermethylated CpG sites covering 330 genes and 1,385 hypomethylated CpG sites covering 321 genes were found in TSCC tissue, compared to the adjacent normal tissue. Furthermore, we chose three candidate genes (FBLN1, ITIH5 and RUNX3) and validated the DNA methylation status by methylation-specific PCR (MS-PCR) and the mRNA expression levels by reverse transcription PCR (RT-PCR). In TSCC tissue, FBLN1 and ITIH5 were shown to be hypermethylated and their expression was found to be decreased, and RUNX3 was shown to be hypomethylated, however, its mRNA expression was found to be increased. In addition, another three genes (BCL2L14, CDCP1 and DIRAS3) were tested by RT-PCR. In TSCC tissue, BCL2L14 and CDCP1 expressions were markedly upregulated, and DIRAS3 expression was significantly downregulated. Our data demonstrated that aberrant DNA methylation is observed in TSCC tissue and plays an important role in the tumorigenesis, development and progression of TSCC. |
| Databáze: | OpenAIRE |
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