Quinolinic acid protects rat cerebellar granule cells from glutamate-induced apoptosis
| Autor: | Yoshitatsu Sei, Anthony S. Basile, Gertrud Goping, Linda H. Fossom, Phil Skolnick |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cerebellum
N-Methylaspartate Excitotoxicity Glutamic Acid Apoptosis Pharmacology Biology medicine.disease_cause Neuroprotection Rats Sprague-Dawley chemistry.chemical_compound Necrosis medicine Excitatory Amino Acid Agonists Animals Neurons General Neuroscience Glutamate receptor Neurotoxicity Quinolinic Acid medicine.disease Rats medicine.anatomical_structure Logistic Models Neuroprotective Agents nervous system chemistry Biochemistry CNQX NMDA receptor Excitatory Amino Acid Antagonists Quinolinic acid |
| Zdroj: | Neuroscience letters. 241(2-3) |
| ISSN: | 0304-3940 |
| Popis: | The effects of quinolinic acid (QUIN) on glutamate-induced excitotoxicity were examined in primary cultures of rat cerebellar granule neurons. Exposing these neurons to QUIN (< or =2.5 mM) in the presence of glucose and Mg2+ had no effect on their viability. Although pretreating neurons with QUIN (10 microM) for 6 h did not reduce necrotic death induced by glutamate exposure in the absence of glucose and Mg2+, QUIN pretreatment significantly suppressed glutamate-induced apoptosis by 68% (as indicated by DNA fragmentation) in cultures containing glucose and Mg2+. Furthermore, the N-methyl-D-aspartate (NMDA) receptor antagonist AP-5 reversed QUIN-induced neuroprotection, while the non-NMDA antagonist CNQX had no effect. This study demonstrates that pathophysiologically relevant concentrations of QUIN can protect neurons from apoptosis mediated via the NMDA receptor. |
| Databáze: | OpenAIRE |
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