Defined and undefined commercial probiotics cultures in the prevention of Salmonella Enteritidis in broilers
| Autor: | Angélica Signor Mendes, Daniel Curvello de Mendonça Müller, Erich Helfer Carvalho, Rosana R. Sikorski, Sabrina Endo Takahashi, Rosângela A.B. Assumpção, Douglas Bonamigo |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Salmonella Salmonella enteritidis Ileum Biology medicine.disease_cause Feed conversion ratio law.invention 03 medical and health sciences Probiotic Cecum law medicine Food science Colony-forming unit lcsh:Veterinary medicine General Veterinary broilers Inoculation Probiotics 0402 animal and dairy science Salmonella Enteritidis 04 agricultural and veterinary sciences 040201 dairy & animal science food safety 030104 developmental biology medicine.anatomical_structure bird health lcsh:SF600-1100 bacterioses control |
| Zdroj: | Pesquisa Veterinária Brasileira v.38 n.2 2018 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Vol 38, Iss 2, Pp 271-276 |
| Popis: | This study aimed to evaluate the efficacy of probiotics from different formations, defined and undefined cultures, applied in the control of Salmonella Enteritidis in broilers, identifying the compositions and states for which the probiotics are more effective. For that, 390 broilers were inoculated orally with 1.00 ml of Salmonella Enteritidis at a concentration of 1.2x109 CFU (Colony Forming Units). The experimental design used was randomized blocks with 5 treatments and 6 replications, totaling 30 boxes with 13 birds/box (13 birds/m2). The treatments were provided via drinking water 1 hour after inoculation, keeping a daily treatment of 12 hours with probiotics, for 3 consecutive days (birds at 1, 2 and 3 days of age). In general, the five treatments conducted were: T1 - Control without probiotic, T2 - Probiotic A (defined culture - lyophilized form, strain 7), T3 - Probiotic B (defined culture - lyophilized form, strain 11), T4 - Probiotic C (undefined culture liquid form), T5 - Probiotic D (undefined culture - liquid form). After treatments, performance was evaluated through average body weight, feed conversion and mortality counting. Microbiological analysis and Salmonella isolation were performed using MPN (Most Probable Number) and selective enrichment technique methods, respectively. Samples of ileum and liver pool, cecal tonsils, cecum, heart and spleen pool were collected at 5 and 31 days of age. No differences were observed on growth performance and isolation of Salmonella Enteritidis (p≥0.05). All probiotics applied were effective on reducing Salmonella Enteritidis colonization in the ileum, cecal tonsils, and cecum at 5 days of life. Probiotics T2 and T5 has shown effectiveness in reducing colonization at 31 days, being considered the most efficient on Salmonella Enteritidis control, for the intestines segments evaluated. It was not possible to affirm which probiotics formation, defined or undefined, is more efficient for Salmonella Enteritidis control. |
| Databáze: | OpenAIRE |
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