MicroRNA Analysis of Human Stroke Brain Tissue Resected during Decompressive Craniectomy/Stroke-Ectomy Surgery
| Autor: | Tamara Roitbak, Andrew P. Carlson, Ron L. Mims, Karen S. SantaCruz, Howard Yonas, Jeremy S. Edwards, William H McKay, Radha Swaminathan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
stroke
malignant hemispheric infarction microRNA next generation microRNA sequencing Decompressive Craniectomy medicine.medical_treatment Brain tissue QH426-470 Bioinformatics Article Cerebrospinal fluid Genetics Medicine Humans Functional studies Stroke Genetics (clinical) business.industry Gene Expression Profiling Brain Computational Biology High-Throughput Nucleotide Sequencing Human brain medicine.disease MicroRNAs medicine.anatomical_structure Decompressive craniectomy Signal transduction business Signal Transduction |
| Zdroj: | Genes Genes, Vol 12, Iss 1860, p 1860 (2021) Genes; Volume 12; Issue 12; Pages: 1860 |
| ISSN: | 2073-4425 |
| Popis: | Background: Signaling pathways mediated by microRNAs (miRNAs) have been identified as one of the mechanisms that regulate stroke progression and recovery. Recent investigations using stroke patient blood and cerebrospinal fluid (CSF) demonstrated disease-specific alterations in miRNA expression. In this study, for the first time, we investigated miRNA expression signatures in freshly removed human stroke brain tissue. Methods: Human brain samples were obtained during craniectomy and brain tissue resection in severe stroke patients with life-threatening brain swelling. The tissue samples were subjected to histopathological and immunofluorescence microscopy evaluation, next generation miRNA sequencing (NGS), and bioinformatic analysis. Results: miRNA NGS analysis detected 34 miRNAs with significantly aberrant expression in stroke tissue, as compared to non-stroke samples. Of these miRNAs, 19 were previously identified in stroke patient blood and CSF, while dysregulation of 15 miRNAs was newly detected in this study. miRNA direct target gene analysis and bioinformatics approach demonstrated a strong association of the identified miRNAs with stroke-related biological processes and signaling pathways. Conclusions: Dysregulated miRNAs detected in our study could be regarded as potential candidates for biomarkers and/or targets for therapeutic intervention. The results described herein further our understanding of the molecular basis of stroke and provide valuable information for the future functional studies in the experimental models of stroke. |
| Databáze: | OpenAIRE |
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