New developments in corneal endothelial cell replacement
Autor: | Sorcha Ní Dhubhghaill, Gerrit R. J. Melles, Marieke Bruinsma, Isabel Dapena, Alina Miron, Martine J. Jager, Daniele Spinozzi, Viridiana Kocaba, Silke Oellerich |
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Přispěvatelé: | Ophtalmology - Eye surgery |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
medicine.medical_specialty
Corneal endothelium genetic structures Endothelium Corneal Diseases/surgery medicine.medical_treatment Corneal Diseases 03 medical and health sciences corneal endothelium 0302 clinical medicine Tissue engineering Cornea Ophthalmology cornea Humans Medicine Cells Cultured Corneal transplantation Endothelium Corneal/transplantation 030304 developmental biology 0303 health sciences business.industry Endothelium Corneal General Medicine Tissue Donors eye diseases Transplantation Endothelial stem cell medicine.anatomical_structure tissue engineering 030221 ophthalmology & optometry Corneal endothelial cell Human medicine Descemet Stripping Endothelial Keratoplasty/methods sense organs business Descemet Stripping Endothelial Keratoplasty transplantation |
Zdroj: | Acta Ophthalmologica Acta Ophthalmologica. WILEY Acta ophthalmologica (Oxford) |
ISSN: | 1755-375X |
Popis: | Corneal transplantation is currently the most effective treatment to restore corneal clarity in patients with endothelial disorders. Endothelial transplantation, either by Descemet membrane endothelial keratoplasty (DMEK) or by Descemet stripping (automated) endothelial keratoplasty (DS(A)EK), is a surgical approach that replaces diseased Descemet membrane and endothelium with tissue from a healthy donor eye. Its application, however, is limited by the availability of healthy donor tissue. To increase the pool of endothelial grafts, research has focused on developing new treatment options as alternatives to conventional corneal transplantation. These treatment options can be considered as either 'surgery-based', that is tissue-efficient modifications of the current techniques (e.g. Descemet stripping only (DSO)/Descemetorhexis without endothelial keratoplasty (DWEK) and Quarter-DMEK), or 'cell-based' approaches, which rely on in vitro expansion of human corneal endothelial cells (hCEC) (i.e. cultured corneal endothelial cell sheet transplantation and cell injection). In this review, we will focus on the most recent developments in the field of the 'cell-based' approaches. Starting with the description of aspects involved in the isolation of hCEC from donor tissue, we then describe the different natural and bioengineered carriers currently used in endothelial cell sheet transplantation, and finally, we discuss the current 'state of the art' in novel therapeutic approaches such as endothelial cell injection. |
Databáze: | OpenAIRE |
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