Simultaneous use of direct and indirect diagnostic techniques in atypical respiratory infections from Chlamydophila pneumoniae and Mycoplasma pneumoniae
| Autor: | Alisa Shurdhi, Salvatore Pignanelli, F. Delucca, Manuela Donati |
|---|---|
| Přispěvatelé: | Pignanelli A., Shurdhi A., Delucca F., Donati M. |
| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Mycoplasma pneumoniae Concordance Clinical Biochemistry medicine.disease_cause law.invention Serology Microbiology law Pneumonia Mycoplasma medicine Pneumonia Bacterial Immunology and Allergy Humans Serologic Tests Polymerase chain reaction business.industry Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) Public Health Environmental and Occupational Health Bacterial pneumonia Reproducibility of Results Hematology Original Articles Chlamydophila pneumoniae medicine.disease Virology respiratory tract diseases Community-Acquired Infections Medical Laboratory Technology Pneumonia Real-time polymerase chain reaction business Bronchoalveolar Lavage Fluid |
| Zdroj: | Journal of clinical laboratory analysis. 23(4) |
| ISSN: | 1098-2825 |
| Popis: | In 2008, 50 samples (BAL), coming from hospital patients, with acute respiratory symptoms have been investigated using two real‐time PCR methods: one assay for the single detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae DNA and one commercially available real‐time duplex PCR assay for the detection of C. pneumoniae and M. pneumoniae DNA. Both techniques used here showed compliant results, with 100% concordance for detection of C. pneumoniae and 98% for detection of M. pneumoniae. The positive results obtained agreed with the clinical suspicion of such infections in some cases and with the presence of IgM specific for C. pneumoniae and M. pneumoniae in all cases of acute infection. J. Clin. Lab. Anal. 23:206–209, 2009. © 2009 Wiley‐Liss, Inc. |
| Databáze: | OpenAIRE |
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