Potential mechanism of GABA secretion in response to the activation of GluK1-containing kainate receptors
| Autor: | Valery P. Zinchenko, Artem M. Kosenkov, Sergei A. Maiorov, S. G. Gaidin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Agonist medicine.drug_class Population Kainate receptor Hippocampal formation Synaptic Transmission 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Receptors Kainic Acid medicine education Receptor gamma-Aminobutyric Acid Neurons education.field_of_study Chemistry General Neuroscience Depolarization General Medicine 030104 developmental biology nervous system Synapses Tetrodotoxin GABAergic Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Research. 171:27-33 |
| ISSN: | 0168-0102 |
| Popis: | Hippocampal GABAergic neurons are subdivided into more than 20 subtypes that are distinguished by features and functions. We have previously described the subpopulation of GABAergic neurons, which can be identified in hippocampal cell culture by the calcium response to the application of domoic acid (DoA), an agonist of kainate receptors (KARs). Here, we investigate the features of DoA-sensitive neurons and their GABA release mechanism in response to KARs activation. We demonstrate that DoA-sensitive GABAergic neurons express GluK1-containing KARs because ATPA, a selective agonist of GluK1-containing receptors, induces the calcium response exclusively in these GABAergic neurons. Our experiments also show that NASPM, previously considered a selective antagonist of calcium-permeable AMPARs, blocks calcium-permeable KARs. We established using NASPM that GluK1-containing receptors of the studied population of GABAergic neurons are calcium-permeable, and their activation is required for GABA release, at least in particular synapses. Notably, GABA release occurs even in the presence of tetrodotoxin, indicating that propagation of the depolarizing stimulus is not required for GABA release in this case. Thus, our data demonstrate that the activation of GluK1-containing calcium-permeable KARs mediates the GABA release by the studied subpopulation of GABAergic neurons. |
| Databáze: | OpenAIRE |
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