Physicochemical and toxicological evaluation of silica nanoparticles suitable for food and consumer products collected by following the EC recommendation
| Autor: | Elise Dumortier, Catia Contado, Olivier Toussaint, Jean-Pascal Piret, Omar Lozano Garcia, Stéphane Lucas, Jorge Mejia |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Surface analysis
food.ingredient Cell Survival Cosmetics 02 engineering and technology 010501 environmental sciences 01 natural sciences Biochemistry NO Analytical Chemistry Nanomaterials food X-ray photoelectron spectroscopy Humans Sedimentation field flow fractionation (SdFFF) Differential centrifugal sedimentation (DCS) Food additives In vitro tests Silica nanoparticles Chemical composition 0105 earth and related environmental sciences Precipitated silica Chromatography Chemistry Photoelectron Spectroscopy Food additive Silicon Dioxide 021001 nanoscience & nanotechnology Fractionation Field Flow Chemical engineering Particle-size distribution Nanoparticles Particle Caco-2 Cells 0210 nano-technology Dispersion (chemistry) |
| Zdroj: | Analytical and Bioanalytical Chemistry. 408:271-286 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-015-9101-8 |
| Popis: | Specific information about the particle size distribution, agglomeration state, morphology, and chemical composition of four silica samples, used as additives in food and in personal care products, were achieved with a combination of analytical techniques. The combined use of differential centrifugal sedimentation (DCS), sedimentation field flow fractionation (SdFFF), and scanning and transmission electron microscopy (SEM and TEM) allows to classify the water dispersed samples as "nanomaterials" according to the EC definition. The mechanical stirring and the ultrasound treatment were compared as dispersion methods. The particle surface chemical composition, determined by particle-induced X-ray emission (PIXE) and X-ray photoelectron spectroscopy (XPS), assessed the different levels of purity between the pyrogenic and the precipitated silica and highlighted particle surface chemical composition modifications in the outer shell when dispersed by mechanical stirring. The potential toxic effects of silica on intestinal Caco-2 cells were investigated using MTS assay and by measuring lactate dehydrogenase (LDH) release and caspases 3/7 activity after 24 h of incubation. No or limited decrease of cell viability was observed for all particles regardless of dispersion procedure, suggesting a relative innocuity of these silica samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink