Nonalcoholic fatty liver disease associated with metabolic syndrome: Influence of liver fibrosis stages on characteristics of very low-density lipoproteins
| Autor: | Eduardo Fassio, Verónica Miksztowicz, Valeria Zago, Estela Alvarez, Cristina Longo, Graciela Landeira, Gisela Gualano, Laura Schreier, Fernando Brites, Diego Martín Lucero, Gabriela Berg |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Liver Cirrhosis Male medicine.medical_specialty Very low-density lipoprotein CIENCIAS MÉDICAS Y DE LA SALUD Nonalcoholic Fatty Liver Disease Liver fibrosis Clinical Biochemistry Very Low-Density Lipoproteins Ciencias de la Salud Lipoproteins VLDL Biochemistry Gastroenterology 03 medical and health sciences 0302 clinical medicine Non-alcoholic Fatty Liver Disease Internal medicine Nonalcoholic fatty liver disease medicine Humans Metabolic Syndrome business.industry Biochemistry (medical) General Medicine Middle Aged medicine.disease Otras Ciencias de la Salud 030104 developmental biology Liver Fibrosis 030211 gastroenterology & hepatology Female Metabolic syndrome business |
| Popis: | Background We evaluated possible changes in VLDLcharacteristics, and metabolic related factors, in MetS-associated NAFLD and accompanying liver fibrosis. Methods We studied 36 MetS patients with biopsy-proven NAFLD (MetS + NAFLD) and 24 MetS without ultrasound NAFLD evidence. Further, MetS + NAFLD was sub-divided according to fibrosis stage into, non-to-moderate (F0–F2, n = 27) and severe (F3–F4, n = 9) fibrosis. We measured: lipid profile, VLDL composition and size (size exclusion-HPLC), CETP and lipoprotein lipase (LPL) activities and adiponectin. Additionally, in MetS + NAFLD type IV collagen 7S domain was measured. Results MetS + NAFLD showed increased VLDL-mass, VLDL particle number, VLDL-triglyceride% and large VLDL-% (p < 0.04). CETP activity tended to increase in MetS + NAFLD (p = 0.058), while LPL activity was unchanged. Moreover, in MetS + NAFLD, adiponectin was decreased (p < 0.001), and negatively correlated with VLDL-mass and VLDL particle number (p < 0.05), independently of insulin-resistance. Within MetS + NAFLD group, despite greater insulin-resistance, patients with severe fibrosis showed lower plasma triglycerides, VLDL-mass, VLDL-triglyceride%, large VLDL-% and CETP activity (p < 0.05), while type IV collagen was increased (p = 0.009) and inversely correlated with large VLDL-% (p = 0.045). Conclusions In MetS, NAFLD is associated with larger and triglyceride over-enriched circulating VLDLs, of greater atherogenicity. However, when NAFLD progresses to severe fibrosis, circulating VLDL features apparently improved, probably due to early alterations in hepatic synthetic function. Fil: Lucero, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Universidad de Buenos Aires. Instituto de Fisiopatología y Bioquímica Clínica; Argentina Fil: Miksztowicz, Verónica Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Universidad de Buenos Aires. Instituto de Fisiopatología y Bioquímica Clínica; Argentina Fil: Gualano, Gisela. Hospital Nacional “Profesor Alejandro Posadas”; Argentina Fil: Longo, Cristina. Hospital Nacional “Profesor Alejandro Posadas”; Argentina Fil: Landeira, Graciela. Hospital Nacional “Profesor Alejandro Posadas”; Argentina Fil: Álvarez, Estela. Hospital Nacional “Profesor Alejandro Posadas”; Argentina Fil: Zago, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Universidad de Buenos Aires. Instituto de Fisiopatología y Bioquímica Clínica; Argentina Fil: Brites, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Universidad de Buenos Aires. Instituto de Fisiopatología y Bioquímica Clínica; Argentina Fil: Berg, Gabriela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Universidad de Buenos Aires. Instituto de Fisiopatología y Bioquímica Clínica; Argentina Fil: Fassio, Eduardo. Hospital Nacional “Profesor Alejandro Posadas”; Argentina Fil: Schreier, Laura Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Universidad de Buenos Aires. Instituto de Fisiopatología y Bioquímica Clínica; Argentina |
| Databáze: | OpenAIRE |
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