Identification and Characterization of Receptor for Cytokine-Induced Neutrophil Chemoattractant-3 on Rat Neutrophils
| Autor: | Takao Kondo, Futoshi Shibata, Hideo Nakagawa, Akemi Ueno, Kazuki Murakami, Maher Al-Mokdad |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Chemokine
Neutrophils medicine.medical_treatment Chemokine CXCL2 Biophysics Biology Biochemistry law.invention Radioligand Assay law medicine Animals Receptors Cytokine Tyrosine Binding site Growth Substances Receptor Molecular Biology Chemotactic Factors Chemotaxis Cell Biology Neutrophil extracellular traps Molecular biology Rats Cross-Linking Reagents Cytokine Mutagenesis Site-Directed Recombinant DNA biology.protein Intercellular Signaling Peptides and Proteins Chemokines CXC |
| Zdroj: | Biochemical and Biophysical Research Communications. 232:562-567 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1997.6136 |
| Popis: | Cytokine-induced neutrophil chemoattractants (CINCs), members of alpha-chemokines, are considered to be major neutrophil chemotactic factors in rats. Recombinant CINC-3/rat macrophage inflammatory protein-2 (MIP-2) having an additional tyrosine residue at the carboxyl terminus (CINC-3-Tyr) was constructed, purified, radiolabeled with 125I, and used for binding studies. The specific binding of 125I-labeled CINC-3-Tyr (125I-CINC-3-Tyr) to rat neutrophils reached a plateau after approximately 60 min at 4 degrees C. This binding of 125I-CINC-3-Tyr could be reversed by adding an excess amount of unlabeled CINC-3-Tyr. Scatchard analysis revealed approximately 12,000 binding sites per cell on rat neutrophils with an apparent Kd value of 120 pM. Chemical cross-linking experiments suggested that the rat neutrophil CINC-3 receptor is a mass of approximately 69 kDa. Taken together, our results strongly suggest that rat neutrophils express a high affinity receptor for CINC-3. |
| Databáze: | OpenAIRE |
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