Effects of the Inhalational Anesthetics Halothane and Sevoflurane on an Experimental Model of Hepatic Injury
| Autor: | Alessandro Menezes, Susi Lauz, Luciane Bicca Mespaque, Emilio Facin, Edna Frasson de Souza Montero, Andrea Fogaça Soubhia |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Methyl Ethers ENFERMIDADES: Hepatopatías Necrosis ANESTESIA: Geral General Anesthesia ANESTÉSICOS: Volátil halotano sevoflurano Hepatopathies Sevoflurane medicine Animals Inhalational Anesthetics Rats Wistar Experimental model business.industry Hypoxia (medical) medicine.disease Rats Disease Models Animal Anesthesiology and Pain Medicine ANESTESIA: General DOENÇAS: Hepatopatites Anesthesia Anesthetics Inhalation Phenobarbital Analysis of variance Chemical and Drug Induced Liver Injury Steatosis medicine.symptom Halothane business medicine.drug |
| Zdroj: | Brazilian Journal of Anesthesiology. 61:591-603 |
| ISSN: | 0034-7094 |
| DOI: | 10.1016/s0034-7094(11)70070-8 |
| Popis: | Summary Background and objectives Hepatic injury after inhalational anesthesia is controversial. Studies have suggested that inhalational agents generate an immune response that can provoke hepatic injury. The objective of this study was to analyze the effects of the inhalational agents halothane and sevoflurane on the liver of rats submitted to hypoxia and reperfusion. Methods Thirty Wistar rats, pretreated with 0.1% phenobarbital for 5 days, with discontinuation of the drug 24 hours before the experiment to cause hepatic injury, were used. Animals were distributed in five groups of six rats each. The control group (C) did not receive any treatment; in the F group, phenobarbital was used to induce hepatic injury; the Hypoxia group was submitted to 14% oxygen (O2); the H group received 1% halothane and 14% O2; and the S group received 2% sevoflurane and 14% O2. Twenty-four hours after exposure to the gases, blood samples were collected to evaluate transaminases (AST and ALT), and liver samples were collected for histological evaluation. Kruskal-Wallis Analysis of Variance and the Newman-Keuls test were used. Results Enzymatic activity mean values of AST (280.33, for halothane, 181, for sevoflurane) and ALT (235 for halothane, and 48.33, for sevoflurane) did not show significant differences, and all groups showed elevated values. Compared to halothane on optical microscopy, sevoflurane had lower indices of morphologic changes with p = 0.045, for steatosis, p = 0.0075, for inflammatory infiltrate, and p = 0.0074, for necrosis. Conclusions Compared to the halothane group, sevoflurane did not show injuries of the liver parenchyma on optical microscopy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|