Zinc Resistance within Swine-Associated Methicillin-Resistant Staphylococcus aureus Isolates in the United States Is Associated with Multilocus Sequence Type Lineage
| Autor: | Tracy L. Nicholson, Peter R. Davies, Jisun Sun, Samantha J. Hau, Timothy S. Frana |
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| Rok vydání: | 2017 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine Swine 030106 microbiology Population Locus (genetics) Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Methicillin 03 medical and health sciences Prevalence medicine Animals Humans education Gene Phylogeny Swine Diseases education.field_of_study Ecology Public and Environmental Health Microbiology SCCmec biochemical phenomena metabolism and nutrition Staphylococcal Infections bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus United States Anti-Bacterial Agents Zinc 030104 developmental biology Staphylococcus aureus Multilocus sequence typing Methicillin Resistance Mobile genetic elements Multilocus Sequence Typing Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 83 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00756-17 |
| Popis: | Zinc resistance in livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) sequence type 398 (ST398) is primarily mediated by the czrC gene colocated with the mecA gene, encoding methicillin resistance, within the type V staphylococcal cassette chromosome mec (SCC mec ) element. Because czrC and mecA are located within the same mobile genetic element, it has been suggested that the use of zinc in feed as an antidiarrheal agent has the potential to contribute to the emergence and spread of methicillin-resistant S. aureus (MRSA) in swine, through increased selection pressure to maintain the SCC mec element in isolates obtained from pigs. In this study, we report the prevalence of the czrC gene and phenotypic zinc resistance in U.S. swine-associated LA-MRSA ST5 isolates, MRSA ST5 isolates from humans with no swine contact, and U.S. swine-associated LA-MRSA ST398 isolates. We demonstrated that the prevalence of zinc resistance in U.S. swine-associated LA-MRSA ST5 isolates was significantly lower than the prevalence of zinc resistance in MRSA ST5 isolates from humans with no swine contact and swine-associated LA-MRSA ST398 isolates, as well as prevalences from previous reports describing zinc resistance in other LA-MRSA ST398 isolates. Collectively, our data suggest that selection pressure associated with zinc supplementation in feed is unlikely to have played a significant role in the emergence of LA-MRSA ST5 in the U.S. swine population. Additionally, our data indicate that zinc resistance is associated with the multilocus sequence type lineage, suggesting a potential link between the genetic lineage and the carriage of resistance determinants. IMPORTANCE Our data suggest that coselection thought to be associated with the use of zinc in feed as an antimicrobial agent is not playing a role in the emergence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST5 in the U.S. swine population. Additionally, our data indicate that zinc resistance is more associated with the multilocus sequence type lineage, suggesting a potential link between the genetic lineage and the carriage of resistance markers. This information is important for public health professionals, veterinarians, producers, and consumers. |
| Databáze: | OpenAIRE |
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