A Novel Bioreactor for the Mechanical Stimulation of Clinically Relevant Scaffolds for Muscle Tissue Engineering Purposes
| Autor: | Silvia Spadoni, Piero G. Pavan, Edoardo Maghin, Martina Piccoli, Silvia Todros |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Muscle tissue Materials science business.product_category Bioengineering Stimulation Strain (injury) lcsh:Chemical technology mechano-transduction lcsh:Chemistry 03 medical and health sciences bioreactor 0302 clinical medicine Bioreactor Diaphragm Mechano-transduction Muscle tissue engineering Numerical modeling medicine Chemical Engineering (miscellaneous) lcsh:TP1-1185 Decellularization Process Chemistry and Technology medicine.disease Clamping Pliers muscle tissue engineering 030104 developmental biology medicine.anatomical_structure numerical modeling lcsh:QD1-999 diaphragm 030220 oncology & carcinogenesis Hyperelastic material business Biomedical engineering |
| Zdroj: | Processes Volume 9 Issue 3 Processes, Vol 9, Iss 474, p 474 (2021) |
| ISSN: | 2227-9717 |
| DOI: | 10.3390/pr9030474 |
| Popis: | Muscular tissue regeneration may be enhanced in vitro by means of mechanical stimulation, inducing cellular alignment and the growth of functional fibers. In this work, a novel bioreactor is designed for the radial stimulation of porcine-derived diaphragmatic scaffolds aiming at the development of clinically relevant tissue patches. A Finite Element (FE) model of the bioreactor membrane is developed, considering two different methods for gripping muscular tissue patch during the stimulation, i.e., suturing and clamping with pliers. Tensile tests are carried out on fresh and decellularized samples of porcine diaphragmatic tissue, and a fiber-reinforced hyperelastic constitutive model is assumed to describe the mechanical behavior of tissue patches. Numerical analyses are carried out by applying pressure to the bioreactor membrane and evaluating tissue strain during the stimulation phase. The bioreactor designed in this work allows one to mechanically stimulate tissue patches in a radial direction by uniformly applying up to 30% strain. This can be achieved by adopting pliers for tissue clamping. Contrarily, the use of sutures is not advisable, since high strain levels are reached in suturing points, exceeding the physiological strain range and possibly leading to tissue laceration. FE analysis allows the optimization of the bioreactor configuration in order to ensure an efficient transduction of mechanical stimuli while preventing tissue damage. |
| Databáze: | OpenAIRE |
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