Motion reduction and multidimensional denoising in Voltage-sensitive Dye imaging
| Autor: | M. Hmila, A. Romero, Daniel J. Strauss, Jacek Szczygielski, J. K. Schubert, Karsten Schwerdtfeger, Philipp Flotho, Lars Haab |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Computer science Movement Noise reduction Image registration Signal-To-Noise Ratio Stimulus (physiology) Somatosensory system Rats Sprague-Dawley Optical imaging Animals Computer vision Voltage-Sensitive Dye Imaging Evoked Potentials Pixel business.industry Somatosensory Cortex Magnetic Resonance Imaging Electric Stimulation Rats Female Artificial intelligence Tibial Nerve Artifacts business Algorithms |
| Zdroj: | EMBC |
| DOI: | 10.1109/embc.2015.7319826 |
| Popis: | Optical Imaging using Voltage-sensitive Dyes is characterized by low fractional changes in fluorescent light intensity upon the application of a stimulus, which leads to slight value differences between pixels on an in-general noisy image sequence. The application of an anisotropic diffusion filtering scheme, in order to contribute to the denoising of the optical images, is proposed as one option to improve its quality and for a better understanding of the physiological processes they represent. We apply an image registration approach to compensate for motion artifacts, such that we do not need to mount a fixed cranial chamber onto the skull. In this work, electrical stimulation to the tibial nerve in a rat model was used to register evoke potentials, imaging the somatosensory cortex of the animal, which was previously stained with the RH1691 dye. |
| Databáze: | OpenAIRE |
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