Differentially Regulated Expression and Function of CD22 in Activated B-1 and B-2 Lymphocytes

Autor: Shozo Izui, R. Michael E. Parkhouse, Thomas Moll, Yves Chicheportiche, Isabelle Semac, Lars Nitschke, Frédéric Lajaunias, Eduardo Martinez-Soria
Rok vydání: 2002
Předmět:
Lipopolysaccharides
Oligodeoxyribonucleotides/pharmacology
Calcium Signaling/immunology
Sialic Acid Binding Ig-like Lectin 2
Interleukin-4/pharmacology
Stimulation
ddc:616.07
Peritoneum/cytology/immunology/metabolism
Lymphocyte Activation
Mice
Adjuvants
Immunologic/pharmacology
immune system diseases
Lectins
hemic and lymphatic diseases
Lymphocyte Activation/ immunology
Immunology and Allergy
B-Lymphocyte Subsets/ immunology/ metabolism
Receptor
Cells
Cultured
Mice
Knockout
Antigens
CD/ biosynthesis/metabolism/physiology
CD22
breakpoint cluster region
Antibodies
Monoclonal
Acquired immune system
Antibodies
Anti-Idiotypic
Up-Regulation
Cell biology
medicine.anatomical_structure
Oligodeoxyribonucleotides
Peritoneum
Signal transduction
Antibodies
Anti-Idiotypic/pharmacology
Immunoglobulin M/immunology
Up-Regulation/immunology
Calcium/metabolism
Immunology
B-Lymphocyte Subsets
Down-Regulation
CpG Islands/immunology
Biology
Adjuvants
Immunologic
Antigens
CD
Antigens
Differentiation
B-Lymphocyte/ biosynthesis/metabolism/physiology
medicine
Animals
Calcium Signaling
CD40 Antigens
B cell
Antigens
CD40/immunology
Lipopolysaccharides/pharmacology
Antibodies
Monoclonal/pharmacology
Antigens
CD22
Mice
Mutant Strains
Antigens
Differentiation
B-Lymphocyte
Mice
Inbred C57BL
Immunoglobulin M
Calcium
CpG Islands
Spleen/cytology/immunology
Interleukin-4
Down-Regulation/immunology
CD5
Cell Adhesion Molecules
Spleen
Zdroj: Journal of Immunology, Vol. 168, No 12 (2002) pp. 6078-6083
Scopus-Elsevier
CIÊNCIAVITAE
ISSN: 1550-6606
0022-1767
DOI: 10.4049/jimmunol.168.12.6078
Popis: CD22 is a B cell-restricted transmembrane protein that apparently controls signal transduction thresholds initiated through the B cell Ag receptor (BCR) in response to Ag. However, it is still poorly understood how the expression of CD22 is regulated in B cells after their activation. Here we show that the expression levels of CD22 in conventional B-2 cells are markedly down-regulated after cross-linking of BCR with anti-IgM mAb but are up-regulated after stimulation with LPS, anti-CD40 mAb, or IL-4. In contrast, treatment with anti-IgM mAb barely modulated the expression levels of CD22 in CD5+ B-1 cells, consistent with a weak Ca2+ response in anti-IgM-treated CD5+ B-1 cells. Moreover, in CD22-deficient mice, anti-IgM treatment did not trigger enhanced Ca2+ influx in CD5+ B-1 cells, unlike CD22-deficient splenic B-2 cells, suggesting a relatively limited role of CD22 in BCR signaling in B-1 cells. In contrast, CD22 levels were markedly down-regulated on wild-type B-1 cells in response to LPS or unmethylated CpG-containing oligodeoxynucleotides. These data indicate that the expression and function of CD22 are differentially regulated in B-1 and conventional B-2 cells, which are apparently implicated in innate and adaptive immunity, respectively.
Databáze: OpenAIRE
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