Pathophysiologic processes have an impact on the plasma metabolomic signature of endometriosis patients
| Autor: | Leonor Puchades-Carrasco, Vicente Payá, Sara Vicente-Muñoz, Antonio Pineda-Lucena, Inmaculada Morcillo, Antonio Pellicer |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Pathology Proton Magnetic Resonance Spectroscopy Endometriosis Disease Gastroenterology Pathogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Internal medicine H-1-NMR spectroscopy medicine Humans Prospective Studies Prospective cohort study plasma Creatinine Academic Medical Centers 030219 obstetrics & reproductive medicine medicine.diagnostic_test business.industry biomarkers Obstetrics and Gynecology Magnetic resonance imaging medicine.disease metabolomics Pathophysiology 030104 developmental biology Reproductive Medicine chemistry Case-Control Studies Female business Biomarkers |
| Zdroj: | FERTILITY AND STERILITY r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) Centro de Investigación Principe Felipe (CIPF) r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) |
| ISSN: | 1556-5653 0015-0282 |
| Popis: | Objective: To evaluate potential variations in the plasma metabolomic profile of endometriosis patients as a consequence of pathophysiologic alterations associated with this disorder. Design: Prospective study. For each subject, a plasma sample was collected after overnight fasting and before surgery. Setting: University medical center. Patient(s): The clinical cohort included 50 endometriosis patients, diagnosed at early (n = 6) and advanced (n = 44) stages of the disease, and 23 healthy women. All volunteers underwent diagnostic laparoscopy to visually confirm the presence or absence of endometriotic lesions. Intervention(s): Metabolomic profiling of plasma samples based on 1H-nuclear magnetic resonance (NMR) spectroscopy in combination with statistical approaches. Main Outcome Measure(s): Comparative identification of metabolites present in plasma from endometriosis patients and healthy women. Result(s): The plasma metabolomic profile of endometriosis patients was characterized by increased concentration of valine, fucose, choline-containing metabolites, lysine/arginine, and lipoproteins and decreased concentration of creatinine compared with healthy women. Metabolic alterations identified in the plasma metabolomic profile of endometriosis patients correlate with pathophysiologic events previously described in the progression of this disease. Conclusion(s): The results highlight the potential of H-1-NMR-based metabolomics to characterize metabolic alterations associated with endometriosis in plasma samples. This information could be useful to get a better understanding of the molecular mechanisms involved in the pathogenesis of endometriosis, thus facilitating the noninvasive diagnosis of this pathology at early stages. (C) 2016 by American Society for Reproductive Medicine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|