Gas chromatography - optical fiber detector for assessment of fatty acids in urban soils
| Autor: | Anabela Cachada, Armando C. Duarte, Ruth Pereira, Teresa Rocha-Santos, Maria E. Pereira, Ana C. Freitas, Teresa S. L. Panteleitchouk, Lurdes I.B. Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Chromatography
Gas Soil test Urban soil Microbial Consortia GC-OF 01 natural sciences Analytical Chemistry law.invention Soil law Flame ionization detector Cities Fatty acids Optical Fibers Soil Microbiology Detection limit chemistry.chemical_classification Chromatography Chemistry 010401 analytical chemistry Fatty Acids Soil chemistry Fatty acid 04 agricultural and veterinary sciences 0104 chemical sciences Linear range Soil water 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Gas chromatography GC-FID Biomarkers |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Fatty acids have been used as biomarkers of the microbial community composition of soils and they are usually separated and quantified by gas-chromatography coupled to a flame ionization detector (GC-FID). The aim of this study was to develop, validate and apply a methodology based on gas chromatography coupled to optical fiber detection (GC-OF) for screening five fatty acids used as indicators of fungal and bacterial communities in urban soils. The performance of the GC-OF methodology (optical fiber detector at 1,550 nm) was evaluated by comparison with the GC-FID methodology and it was found that they were comparable in terms of linear range, detection limit and analytical errors. Besides these similar analytical characteristics, the GC-OF is much cheaper than the GC-FID methodology. Different concentrations were determined for each fatty acid indicator which in turn varied significantly between the soil samples analyzed from Lisbon ornamental gardens. Additionally, the GC-OF showed a great potential as alternative for determination of eleven or more fatty acids in urban soils. published |
| Databáze: | OpenAIRE |
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