A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae
| Autor: | Kukovetz, Kerri, Hertel, Brigitte, Schvarcz, Christopher R., Saponaro, Andrea, Manthey, Mirja, Burk, Ulrike, Greiner, Timo, Steward, Grieg F., Etten, James L. Van, Moroni, Anna, Thiel, Gerhard, Rauh, Oliver |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
K+ channel evolution
Potassium Channels viral K+ channel Phycodnaviridae Lipid Bilayers Sodium lcsh:QR1-502 Genome Viral Mimiviridae Article Ion Channels Sodium Channels lcsh:Microbiology Evolution Molecular Kcv Potassium Viruses Unclassified Amino Acid Sequence Sequence Alignment Sequence Analysis Phylogeny |
| Zdroj: | Viruses, Vol 12, Iss 1107, p 1107 (2020) Viruses Volume 12 Issue 10 |
| ISSN: | 1999-4915 |
| Popis: | Potassium ion (K+) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K+ channels among these alga-infecting viruses has thus far been restricted to members of the family Phycodnaviridae, which are large, double-stranded DNA viruses within the phylum Nucleocytoviricota. Recent sequencing projects revealed that alga-infecting members of Mimiviridae, another family within this phylum, may also contain genes encoding K+ channels. Here we examine the structural features and the functional properties of putative K+ channels from four cultivated members of Mimiviridae. While all four proteins contain variations of the conserved selectivity filter sequence of K+ channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K+ channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K+ over Na+ and a sensitivity to Ba2+. Thus, virus-encoded K+ channels are not limited to Phycodnaviridae but also occur in the members of Mimiviridae. The large sequence diversity among the viral K+ channels implies multiple events of lateral gene transfer. |
| Databáze: | OpenAIRE |
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