Negative SPR Signals during Low Molecular Weight Analyte Recognition
| Autor: | Jérôme Dejeu, Nicolas Spinelli, Hugues Bonnet, Eric Defrancq, Liliane Coche-Guérente, A. Van der Heyden |
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| Přispěvatelé: | Département de Chimie Moléculaire - Ingéniérie et Intéractions BioMoléculaires (DCM - I2BM), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2021 |
| Předmět: |
Analyte
business.industry Chemistry 010401 analytical chemistry 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry [CHIM.ANAL]Chemical Sciences/Analytical chemistry Optoelectronics Sensitivity (control systems) Surface plasmon resonance business ComputingMilieux_MISCELLANEOUS Label free |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2021, 93 (8), pp.4134-4140. ⟨10.1021/acs.analchem.1c00071⟩ |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Surface plasmon resonance (SPR) is a powerful technique for studying biomolecular interactions mainly due to its sensitivity and real-time and label free advantages. While SPR signals are usually positive, only a few studies have reported sensorgrams with negative signals. The aim of the present work is to investigate and to explain the observation of negative SPR signals with the hypothesis that it reflects major changes in ligand conformation resulting from target binding. We demonstrated that these negative unconventional signals were due to the negative complex (ligand/analyte) refractive index increment (RII) deviation from the sum of the RII of the individual entities which counterbalanced the theoretical increase of the signal triggered by the target recognition and the ligand folding. We also found that the conformation change of biomolecules can induce a negative or a positive complex RII deviation depending on its sequence and immobilization mode. |
| Databáze: | OpenAIRE |
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